DataVXAdvanced MaterialsPaper
journal article·2021-10

Graphene Oxide‐Supported Microwell Grids for Preparing Cryo‐EM Samples with Controlled Ice Thickness

Min‐Ho Kang, Junsun Park, Sungsu Kang, Sungho Jeon, Minyoung Lee, Ji‐Yeon Shim, Jeeyoung Lee, Tae Jin Jeon, Min Kyung Ahn, Sung Mi Lee, Ohkyung Kwon, Byung Hyo Kim, Joel R. Meyerson, Min Jae Lee, Kwang‐Il Lim, Soung‐Hun Roh, Won Chul Lee, Jungwon Park

Advanced Materials· Volume 33· Issue 43
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Source abstract

Abstract

Abstract Cryogenic‐electron microscopy (cryo‐EM) is the preferred method to determine 3D structures of proteins and to study diverse material systems that intrinsically have radiation or air sensitivity. Current cryo‐EM sample preparation methods provide limited control over the sample quality, which limits the efficiency and high throughput of 3D structure analysis. This is partly because it is difficult to control the thickness of the vitreous ice that embeds specimens, in the range of nanoscale, depending on the size and type of materials of interest. Thus, there is a need for fine regulation of the thickness of vitreous ice to deliver consistent high signal‐to‐noise ratios for low‐contrast biological specimens. Herein, an advanced silicon‐chip‐based device is developed which has a regular array of micropatterned holes with a graphene oxide (GO) window on freestanding silicon nitride (SixNy). Accurately regulated depths of micropatterned holes enable precise control of vitreous ice thickness. Furthermore, GO window with affinity for biomolecules can facilitate concentration of the sample molecules at a higher level. Incorporation of micropatterned chips with a GO window enhances cryo‐EM imaging for various nanoscale biological samples including human immunodeficiency viral particles, groEL tetradecamers, apoferritin octahedral, aldolase homotetramer complexes, and tau filaments, as well as inorganic materials.
Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced Electron Microscopy Techniques and Applications · Nanopore and Nanochannel Transport Studies · Graphene research and applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Chemical and Biological Engineering, and Institute of Chemical Processes (ICP) Seoul National University Seoul 08826 Republic of Korea

Center for Nanoparticle Research Institute of Basic Science (IBS) Seoul 08826 Republic of Korea

School of Biological Sciences Seoul National University Seoul 08826 Republic of Korea

Department of Mechanical Engineering BK21FOUR ERICA‐ACE Center Hanyang University Ansan Gyeonggi 15588 Republic of Korea

Department of Chemical and Biological Engineering Sookmyung Women's University Seoul 04310 Republic of Korea

Department of Biochemistry and Molecular Biology Seoul National University College of Medicine Seoul 03080 Republic of Korea

National Instrumentation Center for Environmental Management Seoul National University Seoul 08826 Republic of Korea

Department of Materials Science and Engineering Seoul National University Seoul 08826 Republic of Korea

Biomedical Implant Convergence Research Lab Advanced Institutes of Convergence Technology Suwon 16229 Republic of Korea

Department of Organic Materials and Fiber Engineering Soongsil University Seoul 06978 Republic of Korea

Department of Physiology and Biophysics Weill Cornell Medical College of Cornell University New York NY 10065 USA

Funding

Institute for Basic Science · Seoul National University · National Research Foundation · Agency for Defense Development

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