DataVXAdvanced MaterialsPaper
journal article·2024-07

Customized Proteinaceous Nanoformulation for In Vivo Chemical Reprogramming

Huating Chen, Jiangbing Xiang, Yawei Liu, Wei Pi, Hongliang Zhang, Lu Wu, Yiqiong Liu, Shuaifei Ji, Yan Li, Shaoyuan Cui, Kai Liu, Xiaobing Fu, Xiaoyan Sun

Advanced Materials· Volume 36· Issue 28
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Source abstract

Abstract

Abstract Sweat gland (SwG) regeneration is crucial for the functional rehabilitation of burn patients. In vivo chemical reprogramming that harnessing the patient's own cells in damaged tissue is of substantial interest to regenerate organs endogenously by pharmacological manipulation, which could compensate for tissue loss in devastating diseases and injuries, for example, burns. However, achieving in vivo chemical reprogramming is challenging due to the low reprogramming efficiency and an unfavorable tissue environment. Herein, this work has developed a functionalized proteinaceous nanoformulation delivery system containing prefabricated epidermal growth factor structure for on‐demand delivery of a cocktail of seven SwG reprogramming components to the dermal site. Such a chemical reprogramming system can efficiently induce the conversion of epidermal keratinocytes into SwG myoepithelial cells, resulting in successful in situ regeneration of functional SwGs. Notably, in vivo chemical reprogramming of SwGs is achieved for the first time with an impressive efficiency of 30.6%, surpassing previously reported efficiencies. Overall, this proteinaceous nanoformulation provides a platform for coordinating the target delivery of multiple pharmacological agents and facilitating in vivo SwG reprogramming by chemicals. This advancement greatly improves the clinical accessibility of in vivo reprogramming and offers a non‐surgical, non‐viral, and cell‐free strategy for in situ SwG regeneration.
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Research context

Nanomaterials

OpenAlex topics: Graphene and Nanomaterials Applications · Pluripotent Stem Cells Research · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department PLA General Hospital and PLA Medical College State Key Laboratory of Trauma and Chemical Poisoning PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and Regeneration Research Unit of Trauma Care Tissue Repair and Regeneration Chinese Academy of Medical Sciences 2019RU051 Beijing 100048 P. R. China

Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing 100730 P. R. China

State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University 163 Xianlin Avenue Nanjing 210093 China

State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

Department of Nephrology the First Medical Center Chinese PLA General Hospital State Key Laboratory of Kidney Diseases Beijing 100048 P. R. China

Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education) Department of Chemistry Tsinghua University Beijing 100084 China

Funding

National Natural Science Foundation of China · Natural Science Foundation of Jilin Province

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