journal article·2007-10-19Open access

Quantifying the Performance of Protein‐Resisting Surfaces at Ultra‐Low Protein Coverages using Kinesin Motor Proteins as Probes

P. Katira, A. Agarwal, T. Fischer, H.‐Y. Chen, X. Jiang, J. Lahann, H. Hess

Advanced Materials· Volume 19· Issue 20

Abstract

The binding kinetics of fluorescently labeled microtubules to kinesin-coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm–2. This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm–2, enables the characterization of advanced non-fouling coatings, such as (EG)3OH-terminated SAMs and PEGMA with applications in biomedical engineering and bionanotechnology.
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Research context

OpenAlex topics: Micro and Nano Robotics · Microtubule and mitosis dynamics · Advanced Fluorescence Microscopy Techniques

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