In Situ Synthesis of Covalent Organic Frameworks Inside Cells for Triggering Ferroptosis and Immunotherapy
Source abstract
Abstract
ABSTRACT The synthesis of non‐natural polymers inside living systems has become an effective approach for controlling cellular functions and behaviors. However, in situ synthesis of crystalline polymers, such as covalent organic frameworks (COFs), inside cells using dynamic covalent chemistry faces challenges in aqueous environments, crystallization, and functionality. Herein, we present an intralysosomal crystalline polymerization strategy to synthesize COFs inside tumor cells through an acid‐catalyzed imine condensation reaction. The COFs synthesized intracellularly exhibit inherent fluorescence and well‐defined crystalline structures, allowing real‐time monitoring of the synthesis and product characterization. The formation of imine frameworks inside cell lysosomes induces lysosomal damage, activates lysosomal iron, and triggers immunogenic ferroptosis, thus enhancing antitumor immunotherapy. This strategy of intralysosomal crystalline polymerization facilitates the ambient synthesis of COFs, opening avenues for therapeutic polymerization reactions and providing insights into cellular responses to in situ polymer synthesis.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Covalent Organic Framework Applications · Nanoplatforms for cancer theranostics · Click Chemistry and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
State Key Laboratory of Mechanism and Quality of Chinese Medicine Institute of Chinese Medical Sciences University of Macau Taipa Macao China
School of Chemistry and Pharmaceutical Engineering Medical Science and Technology Innovation Center Shandong First Medical University & Shandong Academy of Medical Sciences Jinan China
College of Chemistry Chemical Engineering and Materials Science Collaborative Innovation Center of Functionalized Probes For Chemical Imaging in Universities of Shandong MoE Key Laboratory of Molecular and Nano Probes Shandong Normal University Jinan China
Jiangsu Key Laboratory of Advanced Organic Materials College of Chemistry Nanjing University Nanjing China
Department of Pharmaceutical Sciecnes and MoE Frontiers Science Center for Precision Oncology University of Macau Taipa Macao China
Funding
Science and Technology Development Fund · Universidade de Macau
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