journal article·2024-06

Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines

Xing Lu, Xujiao Zhou, Bin Song, Hong Zhang, Mingrui Cheng, Xingyu Zhu, Yuqi Wu, Haoliang Shi, Binbin Chu, Yao He, Houyu Wang, Jiaxu Hong

Advanced Materials· Volume 36· Issue 26
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Abstract

Abstract Existing tear sensors are difficult to perform multiplexed assays due to the minute amounts of biomolecules in tears and the tiny volume of tears. Herein, the authors leverage DNA tetrahedral frameworks (DTFs) modified on the wireless portable electrodes to effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic and sensitive monitoring of multiple cytokines in human tears. The developed sensors allow the sensitive determination of various dry eye syndrome (DES)‐associated cytokines in human tears with the limit of detection down to 0.1 pg mL−1, consuming as little as 3 mL of tear fluid. Double‐blind testing of clinical DES samples using the developed sensor and commercial ELISA shows no significant difference between them. Compared with single‐biomarker diagnosis, the diagnostic accuracy of this sensor based on multiple biomarkers has improved by ≈16%. The developed system offers the potential for tear sensors to enable personalized and accurate diagnosis of various ocular diseases.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Suzhou Key Laboratory of Nanotechnology and Biomedicine Institute of Functional Nano & Soft Materials & Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO‐CIC) Soochow University Suzhou 215123 China

Department of Ophthalmology and Vision Science, Shanghai Eye, Ear, Nose and Throat Hospital Fudan University Shanghai 200031 China

Macao Translatoinal Medicine Center Macau University of Science and Technology Taipa Macau SAR 999078 China

Institute of Materials Science and Engineering Macau University of Science and Technology Taipa Macau SAR 999078 China

Shanghai Engineering Research Center of Synthetic Immunology Shanghai 200032 China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Higher Education Discipline Innovation Project · Collaborative Innovation Center of Suzhou Nano Science and Technology · Postdoctoral Science Foundation of Jiangsu Province · China Postdoctoral Science Foundation

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