The Guardian of Vision: Intelligent Bacteriophage‐Based Eyedrops for Clinical Multidrug‐Resistant Ocular Surface Infections
Source abstract
Abstract
Abstract Clinical multidrug‐resistant Pseudomonas aeruginosa (MDR‐PA) is the leading cause of refractory bacterial keratitis (BK). However, the reported BK treatment methods lack biosecurity and bioavailability, which usually causes irreversible visual impairment and even blindness. Herein, for BK caused by clinically isolated MDR‐PA infection, armed phages are modularized with the type I photosensitizer (PS) ACR‐DMT, and an intelligent phage eyedrop is developed for combined phagotherapy and photodynamic therapy (PDT). These eyedrops maximize the advantages of bacteriophages and ACR‐DMT, enabling more robust and specific targeting killing of MDR‐PA under low oxygen‐dependence, penetrating and disrupting biofilms, and efficiently preventing biofilm reformation. Altering the biofilm and immune microenvironments alleviates inflammation noninvasively, promotes corneal healing without scar formation, protects ocular tissues, restores visual function, and prevents long‐term discomfort and pain. This strategy exhibits strong scalability, enables at‐home treatment of ocular surface infections with great patient compliance and a favorable prognosis, and has significant potential for clinical application.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bacteriophages and microbial interactions · Ocular Infections and Treatments · Microbial infections and disease research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Ophthalmology Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery TaiKang Center for Life and Medical Sciences Zhongnan Hospital of Wuhan University School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China
College of Biomedical Engineering Sichuan University Chengdu 610065 China
School of Life Science and Engineering Southwest Jiaotong University Chengdu Sichuan 610031 China
Department of Respiratory Diseases Institute of Pediatrics Shenzhen Children's Hospital Shenzhen 518026 China
Department of Burn and Plastic Surgery Shenzhen Key Laboratory of Microbiology in Genomic Modification & Editing and Application Shenzhen Institute of Translational Medicine Shenzhen University Medical School Shenzhen Second People's Hospital The First Affiliated Hospital of Shenzhen University Shenzhen 518035 China
Funding
National Natural Science Foundation of China · National Key Research and Development Program of China · Fundamental Research Funds for the Central Universities
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