DataVXAdvanced MaterialsPaper
journal article·2024-11Open access

In Vivo Optogenetics Based on Heavy Metal‐Free Photon Upconversion Nanoparticles

Masanori Uji, Jumpei Kondo, Chikako Hara‐Miyauchi, Saori Akimoto, Rena Haruki, Yoichi Sasaki, Nobuo Kimizuka, Itsuki Ajioka, Nobuhiro Yanai

Advanced Materials· Volume 36· Issue 46
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Abstract

Abstract Photon upconversion (UC) from red or near‐infrared (NIR) light to blue light is promising for in vivo optogenetics. However, the examples of in vivo optogenetics have been limited to lanthanide inorganic UC nanoparticles, and there have been no examples of optogenetics without using heavy metals. Here the first example of in vivo optogenetics using biocompatible heavy metal‐free TTA‐UC nanoemulsions is shown. A new organic TADF sensitizer, a boron difluoride curcuminoid derivative modified with a bromo group, can promote intersystem crossing to the excited triplet state, significantly improving TTA‐UC efficiency. The TTA‐UC nanoparticles formed from biocompatible surfactants and methyl oleate acquire water dispersibility and remarkable oxygen tolerance. By combining with genome engineering technology using the blue light‐responding photoactivatable Cre‐recombinase (PA‐Cre), TTA‐UC nanoparticles promote Cre‐reporter EGFP expression in neurons in vitro and in vivo. The results open new opportunities toward deep‐tissue control of neural activities based on heavy metal‐free fully organic UC systems.
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Research context

NanomaterialsBiomaterials

OpenAlex topics: Photoreceptor and optogenetics research · Luminescence and Fluorescent Materials · Nanoplatforms for cancer theranostics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Applied Chemistry Graduate School of Engineering and Center for Molecular Systems (CMS) Kyushu University 744 Moto‐oka Nishi‐ku Fukuoka 819‐0395 Japan

Center for Brain Integration Research (CBIR) Tokyo Medical and Dental University (TMDU) 1‐5‐45 Yushima Bunkyo‐ku Tokyo 113‐8510 Japan

Kanagawa Institute of Industrial Science and Technology (KISTEC) 3‐2‐1 Sakato, Takatsu‐ku Kawasaki‐shi Kanagawa 213‐0012 Japan

Research Center for Autonomous Systems Materialogy (AsMAT) Institute of Innovative Research Tokyo Institute of Technology 4259 Nagatsuta‐cho, Midori‐ku Yokohama‐shi Kanagawa 226‐8501 Japan

CREST JST 4‐1‐8 Honcho Kawaguchi‐shi Saitama 332‐0012 Japan

Department of Chemistry Graduate School of Science The University of Tokyo 7‐3‐1 Hongo Bunkyo‐ku Tokyo 113‐0033 Japan

Funding

Takeda Science Foundation · Research Foundation for Opto-Science and Technology · Murata Science Foundation · Japan Society for the Promotion of Science · Japan Science and Technology Corporation

References (48)

Show source-provided references
1. 10.1038/nrn.2017.1510.1038/nrn.2017.15 ↗
2. 10.1016/j.neures.2020.01.00510.1016/j.neures.2020.01.005 ↗
3. 10.1002/adbi.20180023310.1002/adbi.201800233 ↗
4. 10.1002/adhm.20180113210.1002/adhm.201801132 ↗
5. 10.1038/nprot.2009.22610.1038/nprot.2009.226 ↗
6. 10.1093/nsr/nwac00710.1093/nsr/nwac007 ↗
7. 10.1039/C5NR03411F10.1039/C5NR03411F ↗
8. 10.1021/acsnano.5b0638310.1021/acsnano.5b06383 ↗
9. 10.1126/science.aaq114410.1126/science.aaq1144 ↗
10. 10.1002/adma.20180347410.1002/adma.201803474 ↗
11. 10.1002/anie.20191102510.1002/anie.201911025 ↗
12. 10.1016/j.actbio.2021.08.03510.1016/j.actbio.2021.08.035 ↗
13. 10.1002/adfm.20201090710.1002/adfm.202010907 ↗
14. 10.1016/j.jre.2021.10.00310.1016/j.jre.2021.10.003 ↗
15. 10.1002/adma.20220719810.1002/adma.202207198 ↗
16. 10.1021/acsnano.5b0707510.1021/acsnano.5b07075 ↗
17. 10.1021/ja300363810.1021/ja3003638 ↗
18. 10.1021/acsami.9b0662010.1021/acsami.9b06620 ↗
19. 10.1002/anie.20170443010.1002/anie.201704430 ↗
20. 10.1038/s41467-018-05160-110.1038/s41467-018-05160-1 ↗
21. 10.1021/acsami.7b1792910.1021/acsami.7b17929 ↗
22. 10.1016/j.biomaterials.2016.10.00810.1016/j.biomaterials.2016.10.008 ↗
23. 10.1021/ja310426810.1021/ja3104268 ↗
24. 10.1038/nchembio.220510.1038/nchembio.2205 ↗
25. 10.1002/anie.20221534010.1002/anie.202215340 ↗
26. 10.1016/j.cclet.2022.05.02910.1016/j.cclet.2022.05.029 ↗
27. 10.1002/anie.20230309310.1002/anie.202303093 ↗
28. 10.31635/ccschem.022.20210150710.31635/ccschem.022.202101507 ↗
29. 10.1063/1.492691410.1063/1.4926914 ↗
30. 10.1021/acs.chemmater.8b0224710.1021/acs.chemmater.8b02247 ↗
31. 10.1038/s41566-017-0087-y10.1038/s41566-017-0087-y ↗
32. 10.1126/sciadv.160328210.1126/sciadv.1603282 ↗
33. 10.1016/j.orgel.2013.07.01010.1016/j.orgel.2013.07.010 ↗
34. 10.1038/nature1168710.1038/nature11687 ↗
35. 10.1063/1.355890610.1063/1.3558906 ↗
36. 10.1002/adma.20190140410.1002/adma.201901404 ↗
37. 10.1002/adfm.20160250110.1002/adfm.201602501 ↗
38. 10.1039/C7TC00827A10.1039/C7TC00827A ↗
39. 10.1016/0022-2852(69)90225-210.1016/0022-2852(69)90225-2 ↗
40. 10.1021/acsenergylett.0c0115010.1021/acsenergylett.0c01150 ↗
41. 10.1039/c2cp23900k10.1039/c2cp23900k ↗
42. 10.1021/jz300012u10.1021/jz300012u ↗
43. 10.1039/B913243K10.1039/B913243K ↗
44. 10.1103/PhysRevB.78.19511210.1103/PhysRevB.78.195112 ↗
45. 10.1021/acs.jpca.9b0863610.1021/acs.jpca.9b08636 ↗
46. 10.1002/adma.1997009030810.1002/adma.19970090308 ↗
47. 10.1002/ijch.20160011910.1002/ijch.201600119 ↗
48. 10.1038/s41374-020-00482-510.1038/s41374-020-00482-5 ↗