journal article·2024-04

Supramolecular Hydrogel with Ultra‐Rapid Cell‐Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Zhuo Li, Boguang Yang, Zhengmeng Yang, Xian Xie, Zhengnan Guo, Jianyang Zhao, Ruinan Wang, Hao Fu, Pengchao Zhao, Xin Zhao, Guosong Chen, Gang Li, Fuxin Wei, Liming Bian

Advanced Materials· Volume 36· Issue 15
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Abstract

Abstract Cellular energetics plays an important role in tissue regeneration, and the enhanced metabolic activity of delivered stem cells can accelerate tissue repair and regeneration. However, conventional hydrogels with limited network cell adaptability restrict cell–cell interactions and cell metabolic activities. In this work, it is shown that a cell‐adaptable hydrogel with high network dynamics enhances the glucose uptake and fatty acid β‐oxidation of encapsulated human mesenchymal stem cells (hMSCs) compared with a hydrogel with low network dynamics. It is further shown that the hMSCs encapsulated in the high dynamic hydrogels exhibit increased tricarboxylic acid (TCA) cycle activity, oxidative phosphorylation (OXPHOS), and adenosine triphosphate (ATP) biosynthesis via an E‐cadherin‐ and AMP‐activated protein kinase (AMPK)‐dependent mechanism. The in vivo evaluation further showed that the delivery of MSCs by the dynamic hydrogel enhanced in situ bone regeneration in an animal model. It is believed that the findings provide critical insights into the impact of stem cell–biomaterial interactions on cellular metabolic energetics and the underlying mechanisms.
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Research context

OpenAlex topics: 3D Printing in Biomedical Research · Cancer, Hypoxia, and Metabolism · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering The Chinese University of Hong Kong Hong Kong 999077 P. R. China

Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, Department of Orthopaedics and Traumatology The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong 999077 P. R. China

School of Biomedical Sciences and Engineering South China University of Technology Guangzhou 511442 P. R. China

Department of Biomedical Engineering The Hong Kong Polytechnic University Hong Kong 999077 P. R. China

Department of Macromolecular Science Fudan University Shanghai 200433 P. R. China

Department of Orthopedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen 518107 P. R. China

Shenzhen Key Laboratory of Bone Tissue Repair and Translational Research Shenzhen 518107 P. R. China

National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 511442 P. R. China

Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of Technology Guangzhou 511442 P. R. China

Guangdong Provincial Key Laboratory of Biomedical Engineering South China University of Technology Guangzhou 511442 P. R. China

Funding

National Key Research and Development Program of China · Health and Medical Research Fund · Food and Health Bureau · Chinese University of Hong Kong

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