journal article·2024-06Open access

Electroacoustic Responsive Cochlea‐on‐a‐Chip

Yangnan Hu, Jiayue Xing, Hui Zhang, Xinyi Pang, Yabo Zhai, Hong Cheng, Dongyu Xu, Menghui Liao, Yanru Qi, Danqi Wu, Bin Zhang, Lin Cheng, Bo Chu, Chen Zhang, Yuanjin Zhao, Renjie Chai

Advanced Materials· Volume 36· Issue 24

Abstract

Abstract Organ‐on‐chips can highly simulate the complex physiological functions of organs, exhibiting broad application prospects in developmental research, disease simulation, as well as new drug research and development. However, there is still less concern about effectively constructing cochlea‐on‐chips. Here, a novel cochlear organoids‐integrated conductive hydrogel biohybrid system with cochlear implant electroacoustic stimulation (EAS) for cochlea‐on‐a‐chip construction and high‐throughput drug screening, is presented. Benefiting from the superior biocompatibility and electrical property of conductive hydrogel, together with cochlear implant EAS, the inner ear progenitor cells can proliferate and spontaneously shape into spheres, finally forming cochlear organoids with good cell viability and structurally mature hair cells. By incorporating these progenitor cells‐encapsulated hydrogels into a microfluidic‐based cochlea‐on‐a‐chip with culture chambers and a concentration gradient generator, a dynamic and high‐throughput evaluation of inner ear disease‐related drugs is demonstrated. These results indicate that the proposed cochlea‐on‐a‐chip platform has great application potential in organoid cultivation and deafness drug evaluation.
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Research context

OpenAlex topics: Neuroscience and Neural Engineering · Planarian Biology and Electrostimulation · Advanced Sensor and Energy Harvesting Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck Surgery Zhongda Hospital School of Life Sciences and Technology Advanced Institute for Life and Health Jiangsu Province High‐Tech Key Laboratory for Bio‐Medical Research Southeast University Nanjing 210096 China

Co‐Innovation Center of Neuroregeneration Nantong University Nantong 226001 China

College of Food Science and Engineering Nanjing University of Finance and Economics Nanjing 210023 China

School of Medicine Southeast University Nanjing 210009 China

Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu 610000 China

Department of Cell Biology School of Basic Medical Sciences Cheeloo College of Medicine Shandong University Jinan Shandong 250012 P. R. China

State Key Laboratory of Neurology and Oncology Drug Development Nanjing 210000 China

School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and Repair & Beijing Laboratory of Oral Health Capital Medical University Beijing 100069 China

Chinese Institute for Brain Research Beijing 102206 China

Department of Rheumatology and Immunology Nanjing Drum Tower Hospital School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China

Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu 610072 China

Department of Neurology, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology Beijing Institute of Technology Beijing 100081 China

Southeast University Shenzhen Research Institute Shenzhen 518063 China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Science and Technology Department of Sichuan Province · Shenzhen Fundamental Research Program · Natural Science Foundation of Beijing Municipality · China Postdoctoral Science Foundation

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