Site‐Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract
Source abstract
Abstract
The site-specific conjugation of polymers to multiple engineered cysteine residues of a prolyl endopeptidase leads to its stabilization in the gastrointestinal tract of rats, without compromising the activity relative to the native enzyme. The importance of polymer attachment sites is investigated, as well as the significance of polymer structure.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Peptidase Inhibition and Analysis · Diabetes Treatment and Management · Protease and Inhibitor Mechanisms
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemistry and Applied Biosciences Institute of Pharmaceutical Sciences Swiss Federal Institute of Technology Zurich (ETH Zurich) 8093 Zurich Switzerland
Department of Chemistry and Applied Biosciences Laboratory of Organic Chemistry Swiss Federal Institute of Technology Zurich (ETH Zurich) 8093 Zurich Switzerland
EMT Research Center Institut National de la Recherche Scientifique (INRS) J3X 1S2 Varennes Quebec Canada
Funding
Swiss National Science Foundation
References (28)
Show source-provided references
1. 10.1038/nrd239910.1038/nrd2399 ↗
2. 10.1016/S0169-409X(98)00078-710.1016/S0169-409X(98)00078-7 ↗
3. 10.1007/s11095-013-1233-y10.1007/s11095-013-1233-y ↗
4. 10.1016/j.copbio.2011.06.01210.1016/j.copbio.2011.06.012 ↗
5. 10.1038/ncomms652610.1038/ncomms6526 ↗
6. 10.1038/nchembio.179210.1038/nchembio.1792 ↗
7. 10.1038/nchembio78610.1038/nchembio786 ↗
8. 10.1002/adfm.20120222710.1002/adfm.201202227 ↗
9. 10.1038/nrd108810.1038/nrd1088 ↗
10. 10.1126/science.107412910.1126/science.1074129 ↗
11. 10.1073/pnas.110028510810.1073/pnas.1100285108 ↗
12. 10.1073/pnas.040828610210.1073/pnas.0408286102 ↗
13. S.Robic Pat. Appl. WO2007047303 A2 2009.
14. 10.1038/nchem.167510.1038/nchem.1675 ↗
15. 10.1039/c0py90001j10.1039/c0py90001j ↗
16. 10.1016/j.ejpb.2015.04.02510.1016/j.ejpb.2015.04.025 ↗
17. 10.1038/nbt.210810.1038/nbt.2108 ↗
18. 10.1002/(SICI)1097-4636(19980305)39:3<498::AID-JBM22>3.0.CO;2-510.1002/(SICI)1097-4636(19980305)39:3<498::AID-JBM22>3.0.CO;2-5 ↗
19. 10.1021/bm100695410.1021/bm1006954 ↗
20. 10.1529/biophysj.104.04774610.1529/biophysj.104.047746 ↗
21. 10.1021/bi101926u10.1021/bi101926u ↗
22. 10.1021/bi00345a00510.1021/bi00345a005 ↗
23. 10.1002/anie.19900138110.1002/anie.199001381 ↗
24. 10.1002/adma.20080188410.1002/adma.200801884 ↗
25. 10.1016/j.bbapap.2013.06.02010.1016/j.bbapap.2013.06.020 ↗
26. 10.1038/nrgastro.2015.10710.1038/nrgastro.2015.107 ↗
27. 10.1016/j.ejpb.2013.01.01910.1016/j.ejpb.2013.01.019 ↗
28. 10.1016/j.addr.2011.09.01010.1016/j.addr.2011.09.010 ↗