DataVXAdvanced MaterialsPaper
journal article·2024-08Open access

Natural Hydrogels Support Kidney Organoid Generation and Promote In Vitro Angiogenesis

Elena Garreta, Daniel Moya‐Rull, Andrés Marco, Gaia Amato, Asier Ullate‐Agote, Carolina Tarantino, Maria Gallo, David Esporrín‐Ubieto, Alberto Centeno, Amaia Vilas‐Zornoza, Rafael Mestre, María Kalil, Izar Gorroñogoitia, Ane Miren Zaldua, Samuel Sanchez, Laura Izquierdo Reyes, María Eugenia Fernández‐Santos, Felipe Prosper, Nuria Montserrat

Advanced Materials· Volume 36· Issue 34
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Source abstract

Abstract

Abstract To date, strategies aiming to modulate cell to extracellular matrix (ECM) interactions during organoid derivation remain largely unexplored. Here renal decellularized ECM (dECM) hydrogels are fabricated from porcine and human renal cortex as biomaterials to enrich cell‐to‐ECM crosstalk during the onset of kidney organoid differentiation from human pluripotent stem cells (hPSCs). Renal dECM‐derived hydrogels are used in combination with hPSC‐derived renal progenitor cells to define new approaches for 2D and 3D kidney organoid differentiation, demonstrating that in the presence of these biomaterials the resulting kidney organoids exhibit renal differentiation features and the formation of an endogenous vascular component. Based on these observations, a new method to produce kidney organoids with vascular‐like structures is achieved through the assembly of hPSC‐derived endothelial‐like organoids with kidney organoids in 3D. Major readouts of kidney differentiation and renal cell morphology are assessed exploiting these culture platforms as new models of nephrogenesis. Overall, this work shows that exploiting cell‐to‐ECM interactions during the onset of kidney differentiation from hPSCs facilitates and optimizes current approaches for kidney organoid derivation thereby increasing the utility of these unique cell culture platforms for personalized medicine.
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Research context

PolymersBiomaterials

OpenAlex topics: Tissue Engineering and Regenerative Medicine

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Pluripotency for Organ Regeneration Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology (BIST) Carrer de Baldiri i Reixac, 15–21 Barcelona 08028 Spain

University of Barcelona Barcelona 08028 Spain

Regenerative Medicine Program, Centre for Applied Medical Research (CIMA) Universidad de Navarra, Instituto de Investigación Sanitaria de Navarra (IdiSNA) Pamplona 31008 Spain

Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology (BIST) Carrer de Baldiri i Reixac, 10–12 Barcelona 08028 Spain

Instituto de Investigación Biomédica de A Coruña (INIBIC) Complexo Hospitalario Universitario A Coruña (CHUAC), Sergas Universidade da Coruña (UDC) As Xubias A Coruña 15006 Spain

Leartiker S. Coop Xemein Etorbidea 12A Markina‐Xemein 48270 Spain

Catalan Institute for Research and Advanced Studies (ICREA) Passeig de Lluís Companys 23 Barcelona 08010 Spain

Urology Department Hospital Clinic of Barcelona Barcelona 08036 Spain

Department of Cardiology Hospital General Universitario Gregorio Marañón Madrid 28009 Spain

ATMPs Production Unit Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM) Madrid 28009 Spain

Hematology Service and Cell Therapy Unit and Program of Hematology‐Oncology CIMA‐Universidad de Navarra Cancer Center Clínica Universidad de Navarra (CCUN) and Instituto de Investigación Sanitaria de Navarra (IdISNA) Pamplona 31008 Spain

Centro de Investigación Biomedica en Red de Oncología (CIBERONC) and RICORS TERAV Madrid 28029 Spain

Funding

European Research Council · Ministerio de Ciencia e Innovación · European Commission · Fundació la Marató de TV3 · Departament de Salut, Generalitat de Catalunya

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