Engineered Nanomaterials to Potentiate CRISPR/Cas9 Gene Editing for Cancer Therapy
Source abstract
Abstract
Abstract Clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) gene‐editing technology shows promise for manipulating single or multiple tumor‐associated genes and engineering immune cells to treat cancers. Currently, most gene‐editing strategies rely on viral delivery; yet, while being efficient, many limitations, mainly from safety and packaging capacity considerations, hinder the use of viral CRISPR vectors in cancer therapy. In contrast, recent advances in non‐viral CRISPR/Cas9 nanoformulations have paved the way for better cancer gene editing, as these nanoformulations can be engineered to improve safety, efficiency, and specificity through optimizing the packaging capacity, pharmacokinetics, and targetability. In this review, the advance in non‐viral CRISPR delivery is highlighted, and there is a discussion on how these approaches can be potentially used to treat cancers in addressing the aforementioned limitations, followed by the perspectives in designing a proper CRISPR/Cas9‐based cancer nanomedicine system with translational potential.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: CRISPR and Genetic Engineering · RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory of Biomaterials and Translational Medicine Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat‐sen University 600 Tianhe Road Guangzhou 510630 P. R. China
Department of Pharmaceutical Sciences University at Buffalo The State University of New York 3435 Main Street Buffalo NY 14214 USA
Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Sciences 5625 Renmin Street Changchun 130022 P. R. China
Department of Biomedical Engineering Washington University in St. Louis 1 Brookings Drive St. Louis MO 63110 USA
Department of Biomedical Engineering Rosalind and Morris Goodman Cancer Institute McGill University 3655 Promenade Sir William Osler Montreal Quebec H3G 0B1 Canada
Funding
National Key Research and Development Program of China · National Natural Science Foundation of China · Guangdong Provincial Pearl River Talents Program · Youth Innovation Promotion Association of the Chinese Academy of Sciences · China Primary Health Care Foundation
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