DataVXAdvanced MaterialsPaper
journal article·2022-07Open access

Microstructured Hydrogels to Guide Self‐Assembly and Function of Lung Alveolospheres

Claudia Loebel, Aaron I. Weiner, Madeline K. Eiken, Jeremy B. Katzen, Michael P. Morley, Vikram Bala, Fabian L. Cardenas‐Diaz, Matthew D. Davidson, Kazushige Shiraishi, Maria C. Basil, Laura T. Ferguson, Jason R. Spence, Matthias Ochs, Michael F. Beers, Edward E. Morrisey, Andrew E. Vaughan, Jason A. Burdick

Advanced Materials· Volume 34· Issue 28
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Source abstract

Abstract

Abstract Epithelial cell organoids have increased opportunities to probe questions on tissue development and disease in vitro and for therapeutic cell transplantation. Despite their potential, current protocols to grow these organoids almost exclusively depend on culture within 3D Matrigel, which limits defined culture conditions, introduces animal components, and results in heterogenous organoids (i.e., shape, size, composition). Here, a method is described that relies on hyaluronic acid hydrogels for the generation and expansion of lung alveolar organoids (alveolospheres). Using synthetic hydrogels with defined chemical and physical properties, human‐induced pluripotent stem cell (iPSC)‐derived alveolar type 2 cells (iAT2s) self‐assemble into alveolospheres and propagate in Matrigel‐free conditions. By engineering predefined microcavities within these hydrogels, the heterogeneity of alveolosphere size and structure is reduced when compared to 3D culture, while maintaining the alveolar type 2 cell fate of human iAT2‐derived progenitor cells. This hydrogel system is a facile and accessible system for the culture of iPSC‐derived lung progenitors and the method can be expanded to the culture of primary mouse tissue derived AT2 and other epithelial progenitor and stem cell aggregates.
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Research context

Polymers

OpenAlex topics: Neonatal Respiratory Health Research · Tissue Engineering and Regenerative Medicine · Energy Harvesting in Wireless Networks

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Affiliations

Department of Materials Science & Engineering University of Michigan North Campus Research Complex, 2800 Plymouth Rd Ann Arbor MI 48109 USA

Department of Biomedical Engineering University of Michigan Carl A. Gerstacker Building, 2200 Bonisteel Blvd Ann Arbor MI 48109 USA

Department of Bioengineering University of Pennsylvania 240 Skirkanich Hall 210 S. 33rd Street Philadelphia PA 19104 USA

Department of Medicine Lung Biology Institute University of Pennsylvania 3450 Hamilton Walk, Stemmler Hall Philadelphia PA 19104 USA

BioFrontiers Institute and Department of Chemical and Biological Engineering University of Colorado Boulder 3415 Colorado Avenue, 596 UCB Boulder CO 80309 USA

Department of Internal Medicine – Gastroenterology University of Michigan 109 Zina Pitcher Place Ann Arbor MI 48109 USA

Institute of Functional Anatomy Charité – Universitätsmedizin Berlin Campus Charité Mitte, Philippstraße 12 10115 Berlin Germany

School of Veterinary Medicine University of Pennsylvania 3800 Spruce St Philadelphia PA 19104 USA

Funding

National Institutes of Health · National Science Foundation · U.S. Department of Veterans Affairs

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