DataVXAdvanced MaterialsPaper
journal article·2018-05

Protein Bricks: 2D and 3D Bio‐Nanostructures with Shape and Function on Demand

Jianjuan Jiang, Shaoqing Zhang, Zhigang Qian, Nan Qin, Wenwen Song, Long Sun, Zhitao Zhou, Zhifeng Shi, Liang Chen, Xinxin Li, Ying Mao, David L. Kaplan, Stephanie N. Gilbert Corder, Xinzhong Chen, Mengkun Liu, Fiorenzo G. Omenetto, Xiaoxia Xia, Tiger H. Tao

Advanced Materials· Volume 30· Issue 20
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Source abstract

Abstract

Abstract Precise patterning of polymer‐based biomaterials for functional bio‐nanostructures has extensive applications including biosensing, tissue engineering, and regenerative medicine. Remarkable progress is made in both top‐down (based on lithographic methods) and bottom‐up (via self‐assembly) approaches with natural and synthetic biopolymers. However, most methods only yield 2D and pseudo‐3D structures with restricted geometries and functionalities. Here, it is reported that precise nanostructuring on genetically engineered spider silk by accurately directing ion and electron beam interactions with the protein's matrix at the nanoscale to create well‐defined 2D bionanopatterns and further assemble 3D bionanoarchitectures with shape and function on demand, termed “Protein Bricks.” The added control over protein sequence and molecular weight of recombinant spider silk via genetic engineering provides unprecedented lithographic resolution (approaching the molecular limit), sharpness, and biological functions compared to natural proteins. This approach provides a facile method for patterning and immobilizing functional molecules within nanoscopic, hierarchical protein structures, which sheds light on a wide range of biomedical applications such as structure‐enhanced fluorescence and biomimetic microenvironments for controlling cell fate.
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Research context

PolymersNanomaterialsBiomaterials

OpenAlex topics: Silk-based biomaterials and applications · Supramolecular Self-Assembly in Materials · Advanced Materials and Mechanics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China

Department of Mechanical Engineering The University of Texas at Austin Austin TX 78712 USA

State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

School of Graduate Study University of Chinese Academy of Sciences Beijing 100049 China

Department of Neurosurgery Huashan Hospital of Fudan University Wulumuqi Zhong Road 12 Shanghai 200040 China

School of Physical Science and Technology Shanghai Tech University Shanghai 200031 China

Department of Biomedical Engineering Tufts University Medford MA 02155 USA

Department of Physics and Astronomy Stony Brook University Stony Brook NY 11794 USA

Funding

Ministry of Science and Technology of the People's Republic of China · National Science Foundation · Division of Civil, Mechanical and Manufacturing Innovation · National Natural Science Foundation of China · Science and Technology Commission of Shanghai Municipality

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