DataVXAdvanced MaterialsPaper
journal article·2023-02

Engineered Phage with Aggregation‐Induced Emission Photosensitizer in Cocktail Therapy against Sepsis

Ming‐Yu Wu, Luojia Chen, Qingrong Chen, Rui Hu, Xiaoyu Xu, Yifei Wang, Jie Li, Shun Feng, Changjiang Dong, Xiao‐Lian Zhang, Zhiqiang Li, Lianrong Wang, Shi Chen, Meijia Gu

Advanced Materials· Volume 35· Issue 6
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Source abstract

Abstract

Abstract Sepsis, a widely recognized disease, is characterized by multiple pathogen infections. Therefore, it is imperative to develop methods that can efficiently identify and neutralize pathogen species. Phage cocktail therapy utilizes the host specificity of phages to adapt to infect resistant bacteria. However, its low sterilization stability efficiency and lack of imaging units seriously restrict its application. Here, a novel strategy combining the aggregation‐induced emission photosensitizer (AIE‐PS) TBTCP‐PMB with phages through a nucleophilic substitution reaction between benzyl bromide and sulfhydryl groups to remove pathogenic bacteria for sepsis treatment is proposed. This strategy retains the phage's host specificity while possessing AIE‐PS characteristics with a fluorescence imaging function and reactive oxygen species (ROS) for detecting and sterilizing bacteria. This synergetic strategy combining phage cocktail therapy and photodynamic therapy (PDT) shows a strong “1 + 1 > 2” bactericidal efficacy and superior performance in sepsis mouse models with good biocompatibility. Furthermore, the strategy can quickly diagnose blood infections of clinical blood samples. This simple and accurate strategy provides a promising therapeutic platform for rapid pathogen detection and point‐of‐care diagnosis. Moreover, it presents a new method for expanding the library of antibacterial drugs to develop new strain identification and improve infectious disease treatment, thereby demonstrating strong translational potential.
Source: crossref · Quality: baseline

Research context

Biomaterials

OpenAlex topics: Bacteriophages and microbial interactions · Nanoplatforms for cancer theranostics · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Gastroenterology Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery TaiKang Center for Life and Medical Sciences Zhongnan Hospital of Wuhan University School of Pharmaceutical Sciences Wuhan University Wuhan Hubei 430071 P. R. China

Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs School of Life Science and Engineering Southwest Jiaotong University Chengdu Sichuan 610031 P. R. China

Department of Burn and Plastic Surgery Shenzhen Institute of Translational Medicine Health Science Center Shenzhen Second People's Hospital the First Affiliated Hospital of Shenzhen University Shenzhen 518035 P. R. China

Department of Medical Intensive Care Unit Maternal and Child Health Hospital of Hubei Province Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430070 P. R. China

Hubei Province Key Laboratory of Allergy and Immunology Department of Immunology of School of Basic Medical Sciences and Department of Allergy of Zhongnan Hospital Wuhan University Wuhan Hubei 430071 P. R. China

Brain Center Department of Neurosurgery Zhongnan Hospital of Wuhan University School of Pharmaceutical Sciences Wuhan University Wuhan Hubei 430071 P. R. China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Fundamental Research Funds for the Central Universities

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