DataVXAdvanced MaterialsPaper
journal article·2020-07

Ligand Diffusion Enables Force‐Independent Cell Adhesion via Activating α5β1 Integrin and Initiating Rac and RhoA Signaling

Leixiao Yu, Yong Hou, Wenyan Xie, Jose Luis Cuellar Camacho, Chong Cheng, Andrew Holle, Jennifer Young, Britta Trappmann, Weifeng Zhao, Matthias F. Melzig, Elisabetta A. Cavalcanti‐Adam, Changsheng Zhao, Joachim P. Spatz, Qiang Wei, Rainer Haag

Advanced Materials· Volume 32· Issue 29
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Source abstract

Abstract

Abstract Cells reside in a dynamic microenvironment in which adhesive ligand availability, density, and diffusivity are key factors regulating cellular behavior. Here, the cellular response to integrin‐binding ligand dynamics by directly controlling ligand diffusivity via tunable ligand–surface interactions is investigated. Interestingly, cell spread on the surfaces with fast ligand diffusion is independent of myosin‐based force generation. Fast ligand diffusion enhances α5β1 but not αvβ3 integrin activation and initiates Rac and RhoA but not ROCK signaling, resulting in lamellipodium‐based fast cell spreading. Meanwhile, on surfaces with immobile ligands, αvβ3 and α5β1 integrins synergistically initiate intracellular‐force‐based canonical mechanotransduction pathways to enhance cell adhesion and osteogenic differentiation of stem cells. These results indicate the presence of heretofore‐unrecognized pathways, distinct from canonical actomyosin‐driven mechanisms, that are capable of promoting cell adhesion.
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Research context

OpenAlex topics: Cellular Mechanics and Interactions · Cell Adhesion Molecules Research · Force Microscopy Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Chemistry and Biochemistry Freie Universität Berlin Takustr. 3 Berlin 14195 Germany

Institute of Pharmacy Freie Universität Berlin Königin‐Luise‐Str. 2+4 Berlin 14195 Germany

State Key Laboratory of Polymer Materials and Engineering College of Polymer Science and Engineering Sichuan University Chengdu 610065 China

Department of Cellular Biophysics Max Planck Institute for Medical Research Jahnstraße 29 Heidelberg 69120 Germany

Department of Biophysical Chemistry Institute of Physical Chemistry University of Heidelberg Im Neuenheimer Feld 253 Heidelberg 69120 Germany

Max Planck Institute for Molecular Biomedicine Röntgenstr. 20 Münster 48149 Germany

Central Scientific Facility “Cellular Biotechnology” Jahnstr. 29 Heidelberg 69120 Germany

Funding

National Natural Science Foundation of China · State Key Laboratory of Polymer Materials Engineering · Sichuan University · China Scholarship Council · Max-Planck-Gesellschaft

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