Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Hui‐Yu Liu; Ravi Kumar; Chunting Zhong; Saleh Gorji; Liliia Paniushkina; Ramsha Masood; Uwe A. Wittel; Harald Fuchs; Irina Nazarenko; Michael Hirtz

doi:10.1002/adma.202008493

journal article·2021-09Open access

Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Hui‐Yu Liu, Ravi Kumar, Chunting Zhong, Saleh Gorji, Liliia Paniushkina, Ramsha Masood, Uwe A. Wittel, Harald Fuchs, Irina Nazarenko, Michael Hirtz

Advanced Materials· Volume 33· Issue 35

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Source abstract

Abstract

Abstract Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer‐associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer‐associated EVs from complex biofluids (e.g., blood). Here, a novel EV‐capture strategy based on dip‐pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV‐ and cancer‐specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer‐associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies.

Source: crossref · Quality: baseline

Research context

Nanomaterials Biomaterials

OpenAlex topics: Extracellular vesicles in disease · MicroRNA in disease regulation · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF) Karlsruhe Institute of Technology (KIT) Hermann‐von‐Helmholtz‐Platz 1 76344 Eggenstein‐Leopoldshafen Germany

Joint Research Laboratory Nanomaterials (KIT and TUD) at Technische Universität Darmstadt (TUD) Jovanka‐Bontschits‐Str. 2 64287 Darmstadt Germany

Institute for Infection Prevention and Hospital Epidemiology Medical Centre Faculty of Medicine University of Freiburg Breisacher Straße 115 B 79106 Freiburg Germany

Department of General and Visceral Surgery Centre of Surgery Medical Centre Faculty of Medicine University of Freiburg Breisacher Str. 86 79110 Freiburg Germany

Physikalisches Institut & Center for Nanotechnology (CeNTech) Westfälische Wilhelms‐Universität Wilhelm‐Klemm‐Straße 10 48149 Münster Germany

German Cancer Consortium (DKTK) Partner Site Freiburg and German Cancer Research Center (DKFZ) Im Neuenheimer Feld 280 69120 Heidelberg Germany

Funding

H2020 Marie Skłodowska-Curie Actions · Deutscher Akademischer Austauschdienst

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