Wearable Aptalyzer Integrates Microneedle and Electrochemical Sensing for In Vivo Monitoring of Glucose and Lactate in Live Animals

Fatemeh Bakhshandeh; Hanjia Zheng; Nicole G. Barra; Sadegh Sadeghzadeh; Irfani Ausri; Payel Sen; Fatemeh Keyvani; Fasih Rahman; Joe Quadrilatero; Juewen Liu; Jonathan D. Schertzer; Leyla Soleymani; Mahla Poudineh

doi:10.1002/adma.202313743

journal article·2024-08Open access

Wearable Aptalyzer Integrates Microneedle and Electrochemical Sensing for In Vivo Monitoring of Glucose and Lactate in Live Animals

Fatemeh Bakhshandeh, Hanjia Zheng, Nicole G. Barra, Sadegh Sadeghzadeh, Irfani Ausri, Payel Sen, Fatemeh Keyvani, Fasih Rahman, Joe Quadrilatero, Juewen Liu, Jonathan D. Schertzer, Leyla Soleymani, Mahla Poudineh

Advanced Materials· Volume 36· Issue 35

Publisher page Open full text

Source abstract

Abstract

Abstract Continuous monitoring of clinically relevant biomarkers within the interstitial fluid (ISF) using microneedle (MN)‐based assays, has the potential to transform healthcare. This study introduces the Wearable Aptalyzer, an integrated system fabricated by combining biocompatible hydrogel MN arrays for ISF extraction with an electrochemical aptamer‐based biosensor for in situ monitoring of blood analytes. The use of aptamers enables continuous monitoring of a wide range of analytes, beyond what is possible with enzymatic monitoring. The Wearable Aptalyzer is used for real‐time and multiplexed monitoring of glucose and lactate in ISF. Validation experiments using live mice and rat models of type 1 diabetes demonstrate strong correlation between the measurements collected from the Wearable Aptalyzer in ISF and those obtained from gold‐standard techniques for blood glucose and lactate, for each analyte alone and in combination. The Wearable Aptalyzer effectively addresses the limitations inherent in enzymatic detection methods as well as solid MN biosensors and the need for reliable and multiplexed bioanalytical monitoring in vivo.

Source: crossref · Quality: baseline

Research context

Polymers Biomaterials

OpenAlex topics: Advancements in Transdermal Drug Delivery · Electrochemical sensors and biosensors · Spectroscopy Techniques in Biomedical and Chemical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Engineering Physics McMaster University Hamilton Hamilton L8S 4L8 Ontario Canada

Department of Electrical and Computer Engineering University of Waterloo Waterloo N2L 3G1 Canada

Department of Biochemistry and Biomedical Sciences McMaster University Hamilton L8S 4L8 Ontario Canada

Department of Kinesiology and Health Science University of Waterloo Waterloo N2L 3G1 Canada

Department of Chemistry University of Waterloo Waterloo N2L 3G1 Canada

Farncombe Family Digestive Health Research Institute McMaster University Hamilton L8S 4L8 Ontario Canada

Centre for Metabolism Obesity and Diabetes Research McMaster University Hamilton L8S 4L8 Ontario Canada

School of Biomedical Engineering McMaster University Hamilton L8S 4L8 Ontario Canada

Michael G. DeGroote Institute for Infectious Disease Research McMaster University Hamilton L8S 4L8 Ontario Canada

Waterloo Institute for Nanotechnology University of Waterloo Waterloo N2L 3W4 ON Canada

Funding

Canadian Space Agency

References (41)

Show source-provided references

1. 10.1038/s41551-022-00887-110.1038/s41551-022-00887-1 ↗

2. 10.3390/s1905102810.3390/s19051028 ↗

3. 10.3390/chemosensors904008310.3390/chemosensors9040083 ↗

4. 10.1002/adhm.20220236210.1002/adhm.202202362 ↗

5. 10.1016/j.bios.2021.11305410.1016/j.bios.2021.113054 ↗

6. 10.1021/acssensors.2c0103310.1021/acssensors.2c01033 ↗

7. 10.3390/mi1303047810.3390/mi13030478 ↗

8. 10.1039/D0CS00304B10.1039/D0CS00304B ↗

9. Sharma M. · J. Nanosci. Nanotechnol. · 2019 · 9 · 499

10. 10.1021/acsmaterialslett.3c0044110.1021/acsmaterialslett.3c00441 ↗

11. 10.1021/acs.analchem.2c0082910.1021/acs.analchem.2c00829 ↗

12. 10.1002/admi.20220176310.1002/admi.202201763 ↗

13. 10.1002/anie.20230162410.1002/anie.202301624 ↗

14. Reynoso M. · Biosens. Bioelectron. · 2023 · 244 · 956

15. 10.1021/acssensors.9b0112710.1021/acssensors.9b01127 ↗

16. 10.1021/acs.analchem.9b0510910.1021/acs.analchem.9b05109 ↗

17. Dai Y. · ACS Appl. Mater. Interfaces · 2023 · 15

18. 10.1021/jacs.0c0188310.1021/jacs.0c01883 ↗

19. 10.1039/D3LC00210A10.1039/D3LC00210A ↗

20. 10.1002/adma.20210976410.1002/adma.202109764 ↗

21. 10.1021/acsnano.3c0652010.1021/acsnano.3c06520 ↗

22. 10.1021/acs.analchem.6b0322710.1021/acs.analchem.6b03227 ↗

23. 10.1021/jacs.7b0541210.1021/jacs.7b05412 ↗

24. 10.1021/ac902595f10.1021/ac902595f ↗

25. 10.1002/adma.20170224310.1002/adma.201702243 ↗

26. 10.1371/journal.pone.017762810.1371/journal.pone.0177628 ↗

27. 10.1016/j.bios.2022.11491210.1016/j.bios.2022.114912 ↗

28. Zhu D. D. · ACS Appl. Mater. Interfaces · 2023 · 15

29. 10.1002/anie.20221287910.1002/anie.202212879 ↗

30. 10.1126/science.aao675010.1126/science.aao6750 ↗

31. 10.1126/scitranslmed.300709510.1126/scitranslmed.3007095 ↗

32. 10.1039/C9SC01495K10.1039/C9SC01495K ↗

33. 10.1002/adma.20170224310.1002/adma.201702243 ↗

34. 10.1007/s40820-021-00611-910.1007/s40820-021-00611-9 ↗

35. 10.1016/j.bios.2023.11535910.1016/j.bios.2023.115359 ↗

36. 10.1038/s41467-018-07146-510.1038/s41467-018-07146-5 ↗

37. 10.1021/ac060144h10.1021/ac060144h ↗

38. 10.1007/978-1-0716-1803-5_310.1007/978-1-0716-1803-5_3 ↗

39. 10.1021/acs.analchem.2c0082910.1021/acs.analchem.2c00829 ↗

40. 10.1002/btm2.1020110.1002/btm2.10201 ↗

41. Victorious A. · Angew. Chem. · 2022 · 61 · 1