DataVXAdvanced MaterialsPaper
journal article·2019-04

Chirality Controls Mesenchymal Stem Cell Lineage Diversification through Mechanoresponses

Yan Wei, Shengjie Jiang, Mengting Si, Xuehui Zhang, Jinying Liu, Zheng Wang, Cen Cao, Jianyong Huang, Houbing Huang, Lili Chen, Shutao Wang, Chuanliang Feng, Xuliang Deng, Lei Jiang

Advanced Materials· Volume 31· Issue 16
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Source abstract

Abstract

Abstract Biogenesis and tissue development are based on the heterogenesis of multipotent stem cells. However, the underlying mechanisms of stem cell fate specification are unclear. Chirality is one of the most crucial factors that affects stem cell development and is implicated in asymmetrical cell morphology formation; however, its function in heterogeneous cell fate determination remains elusive. In this study, it is reported that the chirality of a constructed 3D extracellular matrix (ECM) differentiates mesenchymal stem cells to diverse lineages of osteogenic and adipogenic cells by providing primary heterogeneity. Molecular analysis shows that left‐handed chirality of the ECM enhances the clustering of the mechanosensor Itgα5, while right‐handed chirality decreases this effect. These differential adhesion patterns further activate distinct mechanotransduction events involving the contractile state, focal adhesion kinase/extracellular signal‐regulated kinase 1/2 cascades, and yes‐associated protein/runt‐related transcription factor 2 nuclear translocation, which direct heterogeneous differentiation. Moreover, theoretical modeling demonstrates that diverse chirality mechanosensing is initiated by biphasic modes of fibronectin tethering. The findings of chirality‐dependent lineage specification of stem cells provide potential strategies for the biogenesis of organisms and regenerative therapies.
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Research context

OpenAlex topics: Cellular Mechanics and Interactions · Microtubule and mitosis dynamics · Developmental Biology and Gene Regulation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Beijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 P. R. China

Department of Dental Materials & Dental Medical Devices Testing Center National Engineering Laboratory for Digital and Material Technology of Stomatology Peking University School and Hospital of Stomatology Beijing 100081 P. R. China

State Key Laboratory of Metal Matrix Composite School of Materials and Science Technology Shanghai Jiaotong University Shanghai 200240 P. R. China

Department of Physics University of Science and Technology Beijing Beijing 100083 P. R. China

Department of Stomatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 P. R. China

Department of Mechanics and Engineering Science College of Engineering Peking University Beijing 100871 P. R. China

Advanced Research Institute of Multidisciplinary Science Beijing Institute of Technology Beijing 100081 P. R. China

CAS Key Laboratory of Bio‐inspired Materials and Interfacial Science CAS Center for Excellence in Nanoscience Technical Institute of Physics and Chemistry Chinese Academy of Sciences Beijing 100190 P. R. China

Funding

National Natural Science Foundation of China · Fok Ying Tong Education Foundation

References (47)

Show source-provided references
1. 10.1016/S0140-6736(17)31366-110.1016/S0140-6736(17)31366-1 ↗
2. Guo M. · Natl. Acad. Sci. USA · 2017 · 114 · 201705179
3. 10.1016/j.stem.2011.06.00810.1016/j.stem.2011.06.008 ↗
4. 10.1038/nmat333910.1038/nmat3339 ↗
5. 10.1016/j.stem.2017.02.00910.1016/j.stem.2017.02.009 ↗
6. 10.1073/pnas.140006511110.1073/pnas.1400065111 ↗
7. 10.1016/j.cell.2017.05.01610.1016/j.cell.2017.05.016 ↗
8. 10.1021/acs.chemrev.6b0035410.1021/acs.chemrev.6b00354 ↗
9. 10.1038/nnano.2017.6210.1038/nnano.2017.62 ↗
10. 10.1021/ar200316n10.1021/ar200316n ↗
11. 10.1021/cr500671p10.1021/cr500671p ↗
12. 10.1038/ncb313710.1038/ncb3137 ↗
13. 10.1021/am504702p10.1021/am504702p ↗
14. Taniguchi K. · Adv. Healthcare Mater. · 2011 · 333 · 339
15. 10.1038/s41467-017-02268-810.1038/s41467-017-02268-8 ↗
16. 10.1007/s12274-016-1239-y10.1007/s12274-016-1239-y ↗
17. 10.1002/adma.20160406210.1002/adma.201604062 ↗
18. 10.1002/adma.20130295810.1002/adma.201302958 ↗
19. 10.1039/B610714A10.1039/B610714A ↗
20. 10.1021/cr050005k10.1021/cr050005k ↗
21. 10.1016/j.biomaterials.2017.03.01410.1016/j.biomaterials.2017.03.014 ↗
22. 10.1038/s41596-018-0005-x10.1038/s41596-018-0005-x ↗
23. 10.1038/s41467-016-0009-610.1038/s41467-016-0009-6 ↗
24. 10.1038/nmat358610.1038/nmat3586 ↗
25. 10.1038/s41467-016-0009-610.1038/s41467-016-0009-6 ↗
26. 10.1038/nmat448910.1038/nmat4489 ↗
27. 10.1016/j.cell.2006.06.04410.1016/j.cell.2006.06.044 ↗
28. 10.1038/nmat440710.1038/nmat4407 ↗
29. 10.1016/j.biomaterials.2013.08.01310.1016/j.biomaterials.2013.08.013 ↗
30. 10.1016/j.biomaterials.2015.08.05310.1016/j.biomaterials.2015.08.053 ↗
31. 10.1038/nmat398010.1038/nmat3980 ↗
32. 10.1083/jcb.20161004210.1083/jcb.201610042 ↗
33. 10.1073/pnas.081233410610.1073/pnas.0812334106 ↗
34. 10.1038/ncb333610.1038/ncb3336 ↗
35. 10.1038/nrm389610.1038/nrm3896 ↗
36. 10.1038/nmat472910.1038/nmat4729 ↗
37. 10.1038/s41563-017-0014-010.1038/s41563-017-0014-0 ↗
38. 10.1038/s41586-018-0444-010.1038/s41586-018-0444-0 ↗
39. 10.1038/nature1013710.1038/nature10137 ↗
40. 10.1021/nn400132510.1021/nn4001325 ↗
41. 10.1021/la035771610.1021/la0357716 ↗
42. 10.1039/c3ra47381c10.1039/c3ra47381c ↗
43. 10.1002/adma.20120043610.1002/adma.201200436 ↗
44. 10.1007/978-3-319-46079-6_1410.1007/978-3-319-46079-6_14 ↗
45. 10.1371/journal.pcbi.100533410.1371/journal.pcbi.1005334 ↗
46. Huang H. J. · Evidence‐Based Complement. Altern. Med. · 2014 · 2014 · 1
47. 10.1073/pnas.101805710810.1073/pnas.1018057108 ↗