journal article·2008-02-04

Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization

J. Li, S. Song, X. Liu, L. Wang, D. Pan, Q. Huang, Y. Zhao, C. Fan

Advanced Materials· Volume 20· Issue 3
Publisher page

Abstract

Seeing is believing. A novel multi- component gold nanoparticle-based nanoprobe is assembled with a thiolated DNA detection probe, horseradish peroxidase (HRP), and bovine serum albumin (BSA). In this nanoprobe, the DNA detection probe is used to construct complexes for “sandwich”-based DNA detection, HRP translates this hybridization event into an enzymatic catalysis-based optical signal, while and BSA acts as a non-specific blocker.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · Gold and Silver Nanoparticles Synthesis and Applications

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