DataVXAdvanced MaterialsPaper
journal article·2019-10Open access

Genetically Programmable Self‐Regenerating Bacterial Hydrogels

Anna M. Duraj‐Thatte, Noémie‐Manuelle Dorval Courchesne, Pichet Praveschotinunt, Jarod Rutledge, Yuhan Lee, Jeffrey M. Karp, Neel S. Joshi

Advanced Materials· Volume 31· Issue 40
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Source abstract

Abstract

Abstract A notable challenge for the design of engineered living materials (ELMs) is programming a cellular system to assimilate resources from its surroundings and convert them into macroscopic materials with specific functions. Here, an ELM that uses Escherichia coli as its cellular chassis and engineered curli nanofibers as its extracellular matrix component is demonstrated. Cell‐laden hydrogels are created by concentrating curli‐producing cultures. The rheological properties of the living hydrogels are modulated by genetically encoded factors and processing steps. The hydrogels have the ability to grow and self‐renew when placed under conditions that facilitate cell growth. Genetic programming enables the gels to be customized to interact with different tissues of the gastrointestinal tract selectively. This work lays a foundation for the application of ELMs with therapeutic functions and extended residence times in the gut.
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Research context

PolymersNanomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Micro and Nano Robotics · Planarian Biology and Electrostimulation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Harvard John A. Paulson School of Engineering and Applied Sciences Harvard University Cambridge MA 02138 USA

Wyss Institute for Biologically Inspired Engineering Harvard University Boston MA 02115 USA

Department of Chemical Engineering McGill University Montreal QC H3A 0C5 Canada

Center for Nanomedicine and Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston MA 02115 USA

Harvard‐MIT Division of Health Sciences and Technology Cambridge MA 02139 USA

Harvard Stem Cell Institute Cambridge MA 02138 USA

Broad Institute of Harvard and MIT Cambridge MA 02142 USA

Funding

National Science Foundation · Korea Institute for Advancement of Technology

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