Aggregation‐Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultradeep In Vivo Bioimaging

Zheng Zheng; Dongyu Li; Zhiyang Liu; Hui‐Qing Peng; Herman H. Y. Sung; Ryan T. K. Kwok; Ian D. Williams; Jacky W. Y. Lam; Jun Qian; Ben Zhong Tang

doi:10.1002/adma.201904799

journal article·2019-11

Aggregation‐Induced Nonlinear Optical Effects of AIEgen Nanocrystals for Ultradeep In Vivo Bioimaging

Zheng Zheng, Dongyu Li, Zhiyang Liu, Hui‐Qing Peng, Herman H. Y. Sung, Ryan T. K. Kwok, Ian D. Williams, Jacky W. Y. Lam, Jun Qian, Ben Zhong Tang

Advanced Materials· Volume 31· Issue 44

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Abstract

Abstract Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high‐spatial‐resolution imaging, and 3D reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third‐harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, a simple method for preparing organic nanocrystals based on an aggregation‐induced emission (AIE) luminogen (DCCN) with bright near‐infrared emission is successfully demonstrated. Aggregation‐induced nonlinear optical effects, including two‐photon fluorescence (2PF), three‐photon fluorescence (3PF), and THG, of DCCN are observed in nanoparticles, especially for crystalline nanoparticles. The nanocrystals of DCCN are successfully applied for 2PF microscopy at 1040 nm NIR‐II excitation and THG microscopy at 1560 nm NIR‐II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy provides much higher spatial resolution and brightness than the 2PF microscopy and can visualize small vessels with diameters of ≈2.7 µm at the deepest depth of 800 µm in a mouse brain. Thus, this is expected to inspire new insights into the development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy.

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Research context

Nanomaterials

OpenAlex topics: Luminescence and Fluorescent Materials · Nanoplatforms for cancer theranostics · Advanced Fluorescence Microscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction Institute for Advanced Study Department of Chemical and Biological Engineering Institute of Molecular Functional Materials Division of Life Science and State Key Laboratory of Molecular Neuroscience The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong China

State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic Research College of Optical Science and Engineering Zhejiang University Hangzhou 310058 China

HKUST‐Shenzhen Research Institute No. 9 Yuexing 1st RD, South Area, Hi‐tech Park Nanshan Shenzhen 518057 China

Center for Aggregation‐Induced Emission SCUT‐HKUST Joint Research Institute State Key Laboratory of Luminescent Materials and Devices South China University of Technology Guangzhou 510640 China

Funding

National Natural Science Foundation of China · Research Grants Council, University Grants Committee · University Grants Committee · Innovation and Technology Commission · National Basic Research Program of China · Natural Science Foundation of Zhejiang Province · National Postdoctoral Program for Innovative Talents

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