Simultaneous Near‐Infrared and Two‐Photon In Vivo Imaging of H₂O₂ Using a Ratiometric Fluorescent Probe based on the Unique Oxidative Rearrangement of Oxonium

A new ratiometric fluorescent H2O2 probe, benzopyrylium–coumarin (BC), is designed by using an oxonium moiety as the unique H2O2 response site. The BC probe exhibits an extremely large emission shift of 221 nm in response to H2O2, and is successfully applied for the simultaneous near-infrared and two-photon imaging of H2O2 in living cells, mouse-liver tissues, and zebrafish. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.