A Surface‐engineered Microfluidic Device for Antibody‐Mediated Negative Selection of High‐Quality Sperm for Assisted Reproduction

ABSTRACT Preparation of spermatozoa with optimal developmental competence remains a challenge in assisted reproduction. Conventional techniques based on sperm motility and morphology fail to adequately remove sperm with DNA damage. Here, we report development of a microfluidic device with a functionalized surface, inspired by the physiological processes of immune cell‐mediated sperm selection in the female reproductive tract. A plasma‐polymerized polyoxazoline (PPOx) film is applied to glass channel slides by deposition of 2‐methyl‐2‐oxazoline, to establish a stable, biocompatible interface confirmed by X‐ray Photoelectron Spectroscopy (XPS), ellipsometry, and sperm culture assays. To selectively eliminate pre‐apoptotic and apoptotic spermatozoa wherein DNA damage is common, anti‐phosphatidylserine (Anti‐PS) antibody is immobilized to the PPOx‐coated surface proximal to the channel slide inlet, while the sperm chemoattractant progesterone is adsorbed near the outlet. To optimize selective functionality, the surface topography is tailored by covalent immobilization of gold nanoparticles and addition of microchannels. Sperm recovered after processing whole liquified semen consistently exhibit high motility and morphology, and low apoptosis‐associated membrane damage, with <1% showing DNA fragmentation. Compared with conventional swim‐up or other microfluidic approaches, the device yields sperm with improved quality, offering a simple one‐step sperm selection strategy with potential for application in human and animal assisted reproduction.