Optimal dilutions of S-PRG filler eluate for experiments on human gingival fibroblasts in vitro
Source abstract
Abstract
The present study attempted to identify the optimal dilution at which at which the effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) filler eluate on human gingival fibroblasts (HGF) may be safely examined in vitro. S-PRG filler is a material that releases six ions and exerts strong caries-suppressing effects. We prepared S-PRG filler eluate in which S-PRG filler and α-MEM were mixed as a medium for HGF. This eluate contains six ions that are released from S-PRG filler. All cells died in proliferation experiments on HGF using S-PRG filler eluate, which demonstrated that unless S-PRG filler eluate was diluted, the ion concentration was strongly cytotoxic. S-PRG filler eluate diluted by 1/100 or more with the addition of 2% or more of FBS was safe for use. We herein successfully established the optimal dilution of S-PRG filler eluate at which HGF may be safely examined in vitro.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Dental Implant Techniques and Outcomes · Dental materials and restorations · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Fixed Prosthodontics and Occlusion, Osaka Dental University
Department of Physiology, Osaka Dental University
Department of Operative Dentistry, Osaka Dental University
Department of Orthodontics, Osaka Dental University
References (30)
Show source-provided references
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