Morphological Changes in Rat Skin Resulting from the Application of Dentin Primer

Morphological changes produced by the application of dentin primer were examined by observing histological responses to the repeated application of aqueous solutions of 35% hydroxyethyl methacrylate and 35% glyceryl methacrylate. One of these two experimental primers was applied to the shaved skin of male Sprague-Dawley rats every eight hours for 21 days. After confirming the disappearance of the inflammation 36 days after the start of the test, the primer application was repeated for an additional 19 days for a total of 55 days. After completion of the primer application, slight hyperkeratosis, which was diagnosed as contact dermatitis, was observed in the epidermis of the treated skin in both the HEMA and the GM groups. These pathological changes were observed as scattered slight hyperkeratosis and slight hypertrophy of the sebaceous glands after both the 21 day and 55 day applications.