Functional evaluation of iPS cell-derived periodontal ligament-like cells
Source abstract
Abstract
Periodontal ligament (PDL) is essential in supporting tooth, remodeling, and regeneration of periodontal tissues; however, donor-derived PDL cells have limited regenerative applications. We previously developed a method to generate PDL-like cells from induced pluripotent stem (iPS) cells using PDL-conditioned medium. In this study, we validated whether iPS-derived PDL cells (iPS-PDL) recapitulate established functions of PDL cells. Morphologically, iPS-PDL resembled primary PDL cells and exhibited a higher proliferative capacity. They expressed PDL-related genes, such as Periostin, Asporin, and Tenascin-C, at levels exceeding those of fibroblasts. Under osteogenic induction, iPS-PDL deposited a mineralized matrix and upregulated the expression of COL1A1, ALPL, OCN, and RUNX2. Ascorbic acid enhanced ALP activity and collagen synthesis, while compressive force induced IL6, IL1β, and COX2 expression, These findings demonstrate that iPS-PDL retain PDL-like functions and are a promising cell source for periodontal regeneration, offering the potential for both therapeutic applications and in vitro modeling of periodontal biology.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Mesenchymal stem cell research · Periodontal Regeneration and Treatments · Pluripotent Stem Cells Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University
Division of Creative and Integrated Medicine, Advanced Medical Research Center, Translational Research Institute for Medical Innovation (TRIMI), Osaka Dental University
Department of Biomaterials, Osaka Dental University
Department of Orthodontics, Osaka Dental University
References (34)
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