journal article·2026-05-25Open access

Effect of an MDP-containing cleaner on bonding of a self-adhesive resin composite to salivary-contaminated root dentin

Shojiro SHIMIZU, Hanemi TSURUTA, Chiaki OKAMOTO, Riho ITO, Masaomi IKEDA, Michael F. BURROW, Toru NIKAIDO, Katsushi OKUYAMA

Dental Materials Journal· Volume 45· Issue 3

Abstract

This study evaluated the effect of an MDP-containing cleaner on the bond strength of a self-adhesive resin composite (SAR) to saliva-contaminated bovine root dentin. Dentin surfaces were ground with #600 SiC and contaminated with artificial saliva for 20 s. Specimens were divided into four groups: water rinse (WA), 10% citric acid-3% ferric chloride solution (10-3), MDP-containing cleaner (KC), and non-contaminated (NC). SAR was applied according to the manufacturer's instructions. After 24 h of water storage at 37°C, μTBS was measured (n=10). KC showed no significant difference in μTBS compared with the NC (p>0.05), while no measurable bond strength was obtained for the WA and 10-3. Mixed failures comprising cohesive failure within dentin, cohesive failure within resin, and adhesive failure at the dentin-resin interface were more frequent in the NC. KC effectively removed contaminants and restored bonding performance. This method offers a promising restorative protocol for challenging clinical settings.
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Research context

OpenAlex topics: Dental materials and restorations · Dental Erosion and Treatment · Endodontics and Root Canal Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Operative Dentistry, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University

Oral Biomedical Engineering, Biomedical Sciences and Engineering, Medical and Dental Sciences and Technology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo

Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital

Asahi University

References (45)

Show source-provided references
1. 1) Tonprasong W, Inokoshi M, Shimizubata M, Yamamoto M, Hatano K, Minakuchi S. Impact of direct restorative dental materials on surface root caries treatment. Evidence based and current materials development: A systematic review. Jpn Dent Sci Rev 2022; 58: 13-30.10.1016/j.jdsr.2021.11.004 ↗
2. 2) Peumans M, Vandormael S, Heeren A, De Munck J, Van Meerbeek B. Six-year clinical performance of a 2-step self-etch adhesive in noncarious cervical lesions. J Adhes Dent 2021; 23: 201-215.
3. 3) Kaida K, Kubo S, Egoshi T, Taira Y. Eight-year clinical evaluation of two types of resin composite in non-carious cervical lesions. Clin Oral Investig 2022; 26: 6327-6337.10.1007/s00784-022-04587-7 ↗
4. 4) Peumans M, Van de Maele E, de Munck J, van Landuyt K, Van Meerbeek B. Fourteen-year clinical performance of a HEMA-free one-step self-etch adhesive in non-carious cervical lesions. J Adhes Dent 2023; 25: 147-158.
5. 5) Van Meerbeek B, Yoshihara K, Van Landuyt K, Yoshida Y, Peumans M. From Buonocore’s pioneering acid-etch technique to self-adhering restoratives. A status perspective of rapidly advancing dental adhesive technology. J Adhes Dent 2020; 22: 7-34.
6. 6) Breschi L. Buonocore memorial lecture 2023: Changing operative mindsets with universal adhesives and cements. Oper Dent 2025; 50: 12-32.10.2341/24-120-B ↗
7. 7) Kitahara S, Shimizu S, Takagaki T, Inokoshi M, Abdou A, Burrow MF, et al. Dentin bonding durability of four different recently introduced self-etch adhesives. Materials (Basel) 2024; 17: 4296.10.3390/ma17174296 ↗
8. 8) Mine A, Nikaido T, Matsumoto M, Takagaki T, Ishida M, Ban S, et al. Status of decontamination methods after using dentin adhesion inhibitors on indirect restorations: An integrative review of 19 publications. Jpn Dent Sci Rev 2021; 57: 147-153.10.1016/j.jdsr.2021.08.001 ↗
9. 9) Shimizu S, Kotake H, Takagaki T, Shinno K, Miyata S, Burrow MF, et al. Evaluation of bonding performance and multi-ion release of S-PRG fillercontaining self-adhesive resin composite. Dent Mater J 2021; 40: 1257-1263.10.4012/dmj.2020-142 ↗
10. 10) Shimizu S, Takagaki T, Ikeda M, Burrow MF, Nikaido T. Dentin bonding performance of the new self-adhesive resin composite. Dent Mater J 2025; 44: 308-314.10.4012/dmj.2024-148 ↗
11. 11) Bourgi R, Cuevas-Suarez CE, Devoto W, Monjarás-Ávila AJ, Monteiro P, Kharma K, et al. Effect of contamination and decontamination methods on the bond strength of adhesive systems to dentin: A systematic review. J Esthet Restor Dent 2023; 35: 1218-1238.10.1111/jerd.13078 ↗
12. 12) Atalay C, Meral E. Does rubbing of universal adhesive reduce the negative effect of saliva on adhesion? J Adhes Dent 2021; 23: 57-65.
13. 13) De-Paula DM, Lomonaco D, Ponte AMP, Cordeiro KE, Moreira MM, Mazzetto SE, et al. Influence of collagen cross-linkers addition in phosphoric acid on dentin biomodification and bonding of an etch-and-rinse adhesive. Dent Mater 2020; 36: e1-e8.10.1016/j.dental.2019.11.019 ↗
14. 14) Manihani AKDS, Mulay S, Beri L, Tandale A, Bhawalkar A, Dalsania R. Comparative evaluation of the effect of two natural collagen cross-linkers on microtensile bond strength of self-etch adhesive system to dentin after contamination with blood and hemostatic agent: An in vitro study. J Conserv Dent Endod 2023; 26: 466-471.
15. 15) Liu T, Xie H, Chen C. A comparison of different cleaning approaches for blood contamination after curing universal adhesives on the dentine surface. Dent Mater 2024; 40: 1786-1797.10.1016/j.dental.2024.07.035 ↗
16. 16) Santos JVDN, Almeida HN, Diniz LBN, Nogueira MA, Junior JFS, Özcan M, et al. Effectiveness of dentin cleaning using air abrasion with aluminum oxide particles on the bond strength of resin-based cements: A systematic review and meta-analysis. J Dent 2025; 161: 105950.10.1016/j.jdent.2025.105950 ↗
17. 17) Coskun ME, Akar T, Tugut F. Airborne-particle abrasion; searching the right parameter. J Dent Sci 2018; 13: 293-300.10.1016/j.jds.2018.02.002 ↗
18. 18) Idono T, Shimizu S, Takagaki T, Burrow MF, Nikaido T. Effect of an MDP-based cleaner on adhesion to dentin contaminated with a temporary luting cement. ODEP 2023; 3: 95-103.
19. 19) Tajiri-Yamada Y, Mine A, Nakatani H, Kawaguchi-Uemura A, Matsumoto M, Hagino R, et al. MDP is effective for removing residual polycarboxylate temporary cement as an adhesion inhibitor. Dent Mater J 2020; 39: 1087-1095.10.4012/dmj.2020-132 ↗
20. 20) Etiennot L, Ordies M, Yao C, Mercelis B, Peumans M, Van Meerbeek B. Effective 10-MDP bonding to saliva-contaminated dentin. J Adhes Dent 2025; 27: 65-74.
21. 21) Perdigão J. Current perspectives on dental adhesion: (1) Dentin adhesion—not there yet. Jpn Dent Sci Rev 2020; 56: 190-207.10.1016/j.jdsr.2020.08.004 ↗
22. 22) Nair P, Ilie N. The long-term consequence of salivary contamination at various stages of adhesive application and clinically feasible remedies to decontaminate. Clin Oral Investig 2020; 24: 4413-4426.10.1007/s00784-020-03307-3 ↗
23. 23) Cengiz T, Ünal M. Comparison of microtensile bond strength and resin-dentin interfaces of two self-adhesive flowable composite resins by using different universal adhesives: Scanning electron microscope study. Microsc Res Tech 2019; 82: 1032-1040.10.1002/jemt.23250 ↗
24. 24) Kaptan A, Oznurhan F. Effects of Er:YAG and Er,Cr:YSGG laser irradiation and adhesive systems on microtensile bond strength of a self-adhering composite. Lasers Med Sci 2023; 38: 41.10.1007/s10103-022-03663-4 ↗
25. 25) Yao Y, Wu D, Cifuentes-Jimenez CC, Sano H, Alvarez-Lloret P, Yamauti M, et al. Comprehensive evaluation of long-term dentin bond strength, water sorption, solubility, and degree of conversion of self-adhesive resin composites. J Adhes Dent 2024; 26: 213-222.
26. 26) Schipper RG, Silletti E, Vingerhoeds MH. Saliva as research material: Biochemical, physicochemical and practical aspects. Arch Oral Biol 2007; 52: 1114-1135.10.1016/j.archoralbio.2007.06.009 ↗
27. 27) Xu F, Laguna L, Sarkar A. Aging-related changes in quantity and quality of saliva: Where do we stand in our understanding? J Texture Stud 2019; 50: 27-35.10.1111/jtxs.12356 ↗
28. 28) Sarkar A, Xu F, Lee S. Human saliva and model saliva at bulk to adsorbed phases—Similarities and differences. Adv Colloid Interface Sci 2019; 273: 102034.10.1016/j.cis.2019.102034 ↗
29. 29) Larsen MJ, Jensen AF, Madsen DM, Pearce EI. Individual variations of pH, buffer capacity, and concentrations of calcium and phosphate in unstimulated whole saliva. Arch Oral Biol 1999; 44: 111-117.10.1016/S0003-9969(98)00108-3 ↗
30. 30) Romero MJ, Nakashima S, Nikaido T, Ichinose S, Sadr A, Tagami J. Inhibition of hydroxyapatite growth by casein, a potential salivary phosphoprotein homologue. Eur J Oral Sci 2015; 123: 288-296.10.1111/eos.12196 ↗
31. 31) Bolme J, Gjerdet NR, Laegreid T. Effect of saliva contamination on the bond strength of single-step and three-step adhesive systems. Eur J Oral Sci 2022; 130: e12838.10.1111/eos.12838 ↗
32. 32) Sattabanasuk V, Shimada Y, Tagami J. Effects of saliva contamination on dentin bond strength using all-in-one adhesives. J Adhes Dent 2006; 8: 311-318.
33. 33) Haralur SB, Alharthi SM, Abohasel SA, Alqahtani KM. Effect of decontamination treatments on micro-shear bond strength between blood-saliva-contaminated post-etched dentin substrate and composite resin. Healthcare (Basel) 2019; 7: 128.10.3390/healthcare7040128 ↗
34. 34) Ülker E, Bilgin S, Kahvecioğlu F, Erkan AI. Effect of saliva decontamination procedures on shear bond strength of a one-step adhesive system. Niger J Clin Pract 2017; 20: 1201-1205.10.4103/1119-3077.187325 ↗
35. 35) Santschi K, Peutzfeldt A, Lussi A, Flury S. Effect of salivary contamination and decontamination on bond strength of two one-step self-etching adhesives to dentin of primary and permanent teeth. J Adhes Dent 2015; 17: 51-57.
36. 36) Gupta N, Tripathi AM, Saha S, Dhinsa K, Garg A. Effect of saliva on the tensile bond strength of different generation adhesive systems: An in-vitro study. J Clin Diagn Res 2015; 9: ZC91-94.10.7860/JCDR/2015/13801.6251 ↗
37. 37) Chen AM, Ekambaram M, Li KC, Cooper PR, Mei ML. A scoping review of the influence of clinical contaminants on bond strength in direct adhesive restorative procedures. J Dent 2024; 145: 104985.10.1016/j.jdent.2024.104985 ↗
38. 38) Burrer P, Dang H, Par M, Attin T, Tauböck TT. Effect of over-etching and prolonged application time of a universal adhesive on dentin bond strength. Polymers (Basel) 2020; 12: 2902.10.3390/polym12122902 ↗
39. 39) Yoshihara K, Hayakawa S, Nagaoka N, Okihara T, Yoshida Y, Van Meerbeek B. Etching efficacy of self-etching functional monomers. J Dent Res 2018; 97: 1010-1016.10.1177/0022034518763606 ↗
40. 40) Yoshihara K, Nagaoka N, Benino Y, Nakamura A, Hara T, Maruo Y, et al. Touch-cure polymerization at the composite cement-dentin interface. J Dent Res 2021; 100: 935-942.10.1177/00220345211001020 ↗
41. 41) Bourgi R, Kharouf N, Cuevas-Suárez CE, Lukomska-Szymanska M, Haikel Y, Hardan L. A literature review of adhesive systems in dentistry: Key components and their clinical applications. Appl Sci 2024; 14: 8111.10.3390/app14188111 ↗
42. 42) Rodrigues RV, Giannini M, Pascon FM, Panwar P, Brömme D, Manso AP, et al. Effect of conditioning solutions containing ferric chloride on dentin bond strength and collagen degradation. Dent Mater 2017; 33: 1093-1102.10.1016/j.dental.2017.07.004 ↗
43. 43) Sebold M, Giannini M, André CB, Sahadi BO, Maravic T, Josic U, et al. Bonding interface and dentin enzymatic activity of two universal adhesives applied following different etching approaches. Dent Mater 2022; 38: 907-923.10.1016/j.dental.2022.03.001 ↗
44. 44) Yamanaka A, Mine A, Shintani A, Aoki-Matsumoto M, Yumitate M, Ban S, et al. Advanced statistical analyses to reduce inconsistencies in bond strength data focused on donor factors: A six-factor analysis using linear mixed and nonlinear regression models. J Prosthodont Res 2024; 68: 585-590.10.2186/jpr.JPR_D_23_00193 ↗
45. 45) de Carvalho MFF, Leijôto-Lannes ACN, Rodrigues MCN, Nogueira LC, Ferraz NKL, Moreira AN, et al. Viability of bovine teeth as a substrate in bond strength tests: A systematic review and meta-analysis. J Adhes Dent 2018; 20: 471-479.