The effect of prime-and-rinse approach using MDP micellar solutions on extrafibrillar demineralization and dentin bond performance

Objective This study investigated the effects of prime-and-rinse approach using 10-methacryloyloxydecyl dihydrogen phosphate (MDP) micellar solutions on extrafibrillar demineralization and dentin bond performance of etch-and-rinse adhesive. Methods The micellar solutions were prepared by adding 15% MDP in two ethanol-aqueous (75:25, 55:45 V/V%) solutions, referring to MDP/EtOH₇₅ and MDP/EtOH₅₅. After mid-coronal dentin surfaces were either etched (control) or conditioned with MDP/EtOH₇₅ and MDP/EtOH₅₅ and rinsed, they were applied with adhesive (Adpter Single Bond 2) in dry- or wet-bonding mode and placed with composite resin (Filtek Z350 XT). They were prepared into multiple micro-beams for micro-tensile bond strengths (MTBS) testing after storage in water for 24 h or subjecting to thermocycling. The other pretreated dentin surfaces were analyzed by TF-XRD, ATR-FTIR, HRTEM, FE-SEM, contact angle measurement and nanoindentation testing. The MTBS data was analyzed with two-way ANOVA followed by LSD post-hoc test. Results MDP/EtOH₇₅ produced significantly greater MTBS values than MDP/EtOH₅₅ and control after thermocycling aging in dry- or wet-bonding mode (P < 0.05). The ATR-FTIR spectrums shows that ratios of phosphate/monomer (1,034 cm^-1/1,716 cm^-1) on MDP/EtOH₇₅-, MDP/EtOH₅₅-treated dentin surfaces are 0.51 and 0.23, respectively. This is confirmed by HRTEM images and SAED pattern that intrafibrillar minerals were mostly preserved after treatment with MDP/EtOH₇₅. MDP/EtOH₇₅ produced significantly higher elastic modulus and nanohardness on pretreated dentin surface than MDP/EtOH₅₅ (P < 0.05). TF-XRD pattern shows some MDP-Ca salts remained on the primed dentin surface. Significance Prime-and-rinse approach using MDP/EtOH₇₅ micellar solution could produce mostly extrafibrillar demineralization, and greatly increase dentin bond durability in dry- or wet-bonding mode.