DataVXAdvanced Functional MaterialsPaper
journal article·2018-11

RNA Fibers as Optimized Nanoscaffolds for siRNA Coordination and Reduced Immunological Recognition

Lauren Rackley, Jaimie Marie Stewart, Jacqueline Salotti, Andrey Krokhotin, Ankit Shah, Justin R. Halman, Ridhima Juneja, Jaclyn Smollett, Lauren Lee, Kyle Roark, Mathias Viard, Mubin Tarannum, Juan Vivero‐Escoto, Peter F. Johnson, Marina A. Dobrovolskaia, Nikolay V. Dokholyan, Elisa Franco, Kirill A. Afonin

Advanced Functional Materials· Volume 28· Issue 48
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Source abstract

Abstract

Abstract RNA is a versatile biomaterial that can be used to engineer nanoassemblies for personalized treatment of various diseases. Despite promising advancements, the design of RNA nanoassemblies with minimal recognition by the immune system remains a major challenge. Here, an approach is reported to engineer RNA fibrous structures to operate as a customizable platform for efficient coordination of siRNAs and for maintaining low immunostimulation. Functional RNA fibers are studied in silico and their formation is confirmed by various experimental techniques and visualized by atomic force microscopy (AFM). It is demonstrated that the RNA fibers offer multiple advantages among which are: i) programmability and modular design that allow for simultaneous controlled delivery of multiple siRNAs and fluorophores, ii) reduced immunostimulation when compared to other programmable RNA nanoassemblies, and iii) simple production protocol for endotoxin‐free fibers with the option of their cotranscriptional assembly. Furthermore, it is shown that functional RNA fibers can be efficiently delivered with various organic and inorganic carriers while retaining their structural integrity in cells. Specific gene silencing triggered by RNA fibers is assessed in human breast cancer and melanoma cell lines, with the confirmed ability of functional fibers to selectively target single nucleotide mutations.
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Research context

NanomaterialsBiomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · RNA and protein synthesis mechanisms

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Nanoscale Science Program Department of Chemistry University of North Carolina at Charlotte Charlotte NC 28223 USA

Department of Bioengineering University of California Riverside CA 92521 USA

Mouse Cancer Genetics Program Center for Cancer Research National Cancer Institute Frederick MD 21702 USA

Department of Biochemistry and Biophysics University of North Carolina Chapel Hill NC 27514 USA

Nanotechnology Characterization Lab Cancer Research Technology Program Frederick National Laboratory for Cancer Research Sponsored by the National Cancer Institute Frederick MD 21702 USA

Cancer and Inflammation Program Leidos Biomedical Research Inc. Frederick National Laboratory for Cancer Research Frederick MD 21702 USA

Department of Pharmacology Department of Biochemistry & Molecular Biology Penn State College of Medicine Hershey PA 17033 USA

Department of Mechanical Engineering University of California Riverside CA 92521 USA

The Center for Biomedical Engineering and Science University of North Carolina at Charlotte Charlotte NC 28223 USA

Funding

National Science Foundation · National Institutes of Health · National Cancer Institute · Frederick National Laboratory for Cancer Research

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