Label‐Free and Continuous‐Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids
Source abstract
Abstract
In this study, a label‐free, low‐cost, and fast ferrohydrodynamic cell separation scheme is demonstrated using HeLa cells (an epithelial cell line) and red blood cells. The separation is based on cell size difference, and conducted in a custom‐made biocompatible ferrofluid that retains the viability of cells during and after the assay for downstream analysis. The scheme offers moderate‐throughput (≈106 cells h−1 for a single channel device) and extremely high recovery rate (>99%) without the use of any label. It is envisioned that this separation scheme will have clinical applications in settings where rapid cell enrichment and removal of contaminating blood will improve efficiency of screening and diagnosis such as cervical cancer screening based on mixed populations in exfoliated samples.
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Research context
OpenAlex topics: Microfluidic and Bio-sensing Technologies · Characterization and Applications of Magnetic Nanoparticles · Microfluidic and Capillary Electrophoresis Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemistry The University of Georgia Athens GA 30602 USA
College of Engineering The University of Georgia 220 Riverbend Road, Room 166 Athens GA 30602 USA
Department of Physics and Astronomy Clemson University Clemson SC 29634 USA
Department of Biochemistry and Molecular Biology Complex Carbohydrate Research Center The University of Georgia Athens GA 30602 USA
Department of Pathology College of Veterinary Medicine The University of Georgia Athens GA 30602 USA
Chronic Viral Diseases Branch Division of High‐Consequence Pathogens and Pathology National Center for Emerging and Zoonotic Infectious Diseases Pathology Centers for Disease Control and Prevention Atlanta GA 30329 USA
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