Nanoelectrodes for intracellular measurements of reactive oxygen and nitrogen species in single living cells
Source abstract
Abstract
Reactive oxygen and nitrogen species (ROS and RNS) play important roles in various physiological processes (e.g., phagocytosis) and pathological conditions (e.g., cancer). The primary ROS/RNS, viz., hydrogen peroxide, peroxynitrite ion, nitric oxide, and nitrite ion, can be oxidized at different electrode potentials and therefore detected and quantified by electroanalytical techniques. Nanometer-sized electrochemical probes are especially suitable for measuring ROS/RNS in single cells and cellular organelles. In this article, we survey recent advances in localized measurements of ROS/RNS inside single cells and discuss several methodological issues, including optimization of nanoelectrode geometry, precise positioning of an electrochemical probe inside a cell, and interpretation of electroanalytical data.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Analytical Chemistry and Sensors · Electrochemical Analysis and Applications · Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (52)
Show source-provided references
1. Optical method10.1146/annurev.bb.20.060191.000245 ↗
2. Davies · 1995
3. Halliwell · 1999
4. Biochemistry of oxidative stress10.1002/anie.198610581 ↗
5. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology10.1152/physrev.00044.2005 ↗
6. Electrochemical monitoring of single cell secretion: vesicular exocytosis and oxidative stress10.1021/cr068062g ↗
7. Detection of oxidative stress responses at the level of a single human fibroblast10.1021/ac00115a004 ↗
8. Analysis of individual biochemical events based on artificial synapses using ultramicroelectrodes: cellular oxidative burst10.1039/b001448f ↗
9. Zeptomole voltammetric detection and electron-transfer rate measurements using platinum electrodes of nanometer dimensions10.1021/ac0342931 ↗
10. Monitoring dopamine release from single living vesicles with nanoelectrodes10.1021/ja050385r ↗
11. Development of Au disk nanoelectrode down to 3 nm in radius for detection of dopamine release from a single cell10.1021/ac5042999 ↗
12. Nanoelectrode for amperometric monitoring of individual vesicular exocytosis inside single synapses10.1002/anie.201404744 ↗
13. Real-time monitoring of discrete synaptic release events and excitatory potentials within self-reconstructed neuro-muscular junctions10.1002/anie.201503801 ↗
14. Intracellular analysis with an immobilized-enzyme glucose electrode having a 2-μm diameter and subsecond response times10.1021/ja00019a049 ↗
15. Nanokit for single-cell electrochemical analyses10.1073/pnas.1609618113 ↗
16. Nanoelectrodes for determination of reactive oxygen and nitrogen species inside murine macrophages10.1073/pnas.1201552109 ↗
17. Quantitative measurement of transmitters in individual vesicles in the cytoplasm of single cells with nanotip electrodes10.1002/anie.201504839 ↗
18. Electrochemical nanoneedle biosensor based on multiwall carbon nanotube10.1021/ac0508595 ↗
19. Fabrication of functional micro- and nanoneedle electrodes using a carbon nanotube template and electrodeposition10.1186/1556-276X-6-306 ↗
20. Individual nanotube-based needle nanoprobes for electrochemical studies in picoliter microenvironments10.1021/nn700171x ↗
21. Single tungsten nanowires as pH sensitive electrodes10.1016/j.elecom.2008.05.008 ↗
22. Subcellular neural probes from single-crystal gold nanowires10.1021/nn5024522 ↗
23. Electro-triggering and electrochemical monitoring of dopamine exocytosis from a single cell by using ultrathin electrodes based on Au nanowires10.1039/C5NR06021D ↗
24. Murad · 1998
25. Local force and geometry sensing regulate cell functions10.1038/nrm1890 ↗
26. Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus10.1038/nrm2594 ↗
27. In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress10.1039/c0cp00398k ↗
28. Local detection of mechanically induced ATP release from bone cells with ATP microbiosensors10.1016/j.bios.2013.01.008 ↗
29. Stretchable electrochemical sensor for real-time monitoring of cells and tissues10.1002/anie.201601276 ↗
30. Stretchable electrochemical sensor for inducing and monitoring cell mechanotransduction in real-time
31. Conductive polymer-coated carbon nanotubes to construct stretchable and transparent electrochemical sensors10.1021/acs.analchem.6b04616 ↗
32. Real-time amperometric analysis of reactive oxygen and nitrogen species released by single immunostimulated macrophages10.1002/cbic.200700746 ↗
33. Furthermore, O2 reduction itself would generate much higher O2•− and H2O2 concentrations than those present in or near the cells and alter the relative distributions of endogenous ROS/RNS. For this reason, relying on ROS/RNS oxidation waves is mandatory [6-8].
34. Highly sensitive platinum-black coated platinum electrodes for electrochemical detection of hydrogen peroxide and nitrite in microchannel10.1002/elan.201200456 ↗
35. This is precisely the reason why freshly prepared Pt-black electrodes are used for conductimetric measurements.
36. Carbon pipette-based electrochemical nanosampler10.1021/ac403547b ↗
37. Direct electrochemical measurements of reactive oxygen and nitrogen species in nontransformed and metastatic human breast cells10.1021/jacs.7b06476 ↗
38. Electrochemical measurements of reactive oxygen and nitrogen species inside single phagolysosomes of living macrophages10.1021/jacs.9b01217 ↗
39. Real-Time intracellular measurements of ROS and RNS in living cells with single core-shell nanowire electrodes10.1002/anie.201707187 ↗
40. Electrochemical monitoring of ROS/RNS homeostasis within individual phagolysosomes inside single macrophages10.1002/anie.201902734 ↗
41. Protein kinase C and cancer: what we know and what we do not10.1038/onc.2013.524 ↗
42. Monitoring in real time with a microelectrode the release of reactive oxygen and nitrogen species by a single macrophage stimulated by its membrane mechanical depolarization10.1002/cbic.200500359 ↗
43. Diffusion of peroxynitrite across erythrocyte membranes10.1073/pnas.95.7.3566 ↗
44. Lysosomal destabilization during macrophage damage induced by cholesterol oxidation products10.1016/S0891-5849(99)00220-8 ↗
45. The reactive oxygen species in macrophage polarization: reflecting its dual role in progression and treatment of human diseases10.1155/2016/2795090 ↗
46. Role of nitric oxide synthesis in macrophage antimicrobial activity10.1016/0952-7915(91)90079-G ↗
47. The role of nitric oxide in metabolic regulation of dendritic cell immune function10.1016/j.canlet.2017.10.032 ↗
48. Differential patterns of peroxynitrite mediated apoptosis in proximal tubular epithelial cells following ATP depletion recovery10.1007/s10495-008-0196-7 ↗
49. Characterizing the catecholamine content of single mammalian vesicles by collision–adsorption events at an electrode10.1021/ja512972f ↗
50. On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes10.1039/C6FD00102E ↗
51. Electrochemical resistive-pulse sensing10.1021/jacs.9b10329 ↗
52. Resistive-pulse sensing inside single living cells10.1021/jacs.9b13796 ↗