journal article·2025-08

Multiphase Mineralized Collagen Scaffold for Infected Bone Regeneration Through Oxidative Metabolism Modulation

Qibo Wang, Zixin Li, Yifan Zhang, Houzuo Guo, Liyuan Chen, He Zhang, Hangbo Liu, Chang Li, Shiying Zhang, Xinmeng Shi, Lifang Feng, Mengchun Qi, Dan Luo, Ping Di, Yan Liu

Advanced Functional Materials· Volume 35· Issue 32
Publisher page

Abstract

Abstract Bone defects, especially infected ones, present significant clinical challenges. Bone tissue engineering materials exhibiting remarkable osteogenic and antimicrobial properties have increasingly been focused upon to tackle such challenges. Herein, the preparation of a multiphase mineralized collagen scaffold with copper sulfide nanoparticles (mp‐MCS) is described, which promotes osteogenesis while providing excellent antibacterial properties. mp‐MCS exhibited a bone‐like hierarchical nanostructure and modulus, with rapid copper‐ion release in the first 24 h, followed by a slower sustained release. In vitro experiments, mp‐MCS supported cell viability and enhanced osteoblastic differentiation of bone marrow‐derived mesenchymal stem cells. Mechanistically, mp‐MCS boosted mitochondrial oxidative phosphorylation to facilitate energy production via controlled release of copper ions, which serve as a cofactor for enzymes in the electron transport chain, particularly cytochrome c oxidase. Additionally, the mimetic enzyme activities of the scaffold generated reactive oxygen species, disrupting cell membranes and metabolism in bacteria, leading to bacterial death and strong antibacterial effects. Genetic sequencing confirmed that mp‐MCS interfered with bacterial physiology and key metabolic pathways. In vivo, studies using rat calvarial bone‐defect models showed significant bone regeneration under both infected and non‐infected conditions. Overall, mp‐MCS shows great promise as a clinical solution for repairing bone defects, particularly in infection‐complicated cases.
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Research context

OpenAlex topics: Bone Tissue Engineering Materials · Bone and Dental Protein Studies · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Central Laboratory Department of Orthodontics Peking University School and Hospital for Stomatology National Center for Stomatology National Clinical Research Center for Oral Diseases National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials Beijing Advanced Center of Cellular Homeostasis and...

Department of Oral Implantology Peking University School and Hospital for Stomatology National Center for Stomatology National Clinical Research Center for Oral Diseases Beijing Key Laboratory of Digital Stomatology Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health NMPA Key Laboratory for Dental Materials National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing 100081 P. R. China

Department of Stomatology Peking University People's Hospital Beijing 100044 P. R. China

Department of Oral Maxillofacial Surgery College of Stomatology North China University of Science and Technology Tangshan Hebei 063210 P. R. China

Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 101400 P. R. China

Funding

National Basic Research Program of China · Innovative Research Team of High-level Local University in Shanghai · National Ten Thousand Talent Program · Natural Science Foundation of Beijing Municipality · National Natural Science Foundation of China

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