Engineering electrochemical interface for biomolecular sensing

Abstract Sensing interfaces where molecular recognition processes occur plays a central role in biosensors, which directly affect the biosensing sensitivity, specificity, stability, and response dynamics. However, the target accessibility of conventional sensing interfaces is limited, resulting in low molecular recognition efficiency. Engineering the sensing interfaces of biosensors provides an effective strategy to improve the performance of the biosensors. In this review, we summarize recent advances during the past two years in the development of biosensing interface engineering for enhanced electrochemical sensing. Three types of emerging engineering approaches, including constructing nanostructured sensing interfaces, molecule-mediated interfacial regulation, and functionalizing the interfaces with self-assembled DNA nanostructures, especially framework nucleic acid, are discussed. Also, the remaining challenges and opportunities are outlined for the important research area.