Electrochemical analysis of nucleic acids as potential cancer biomarkers
Source abstract
Abstract
Abstract In recent years, a huge progress has been made regarding the development of electrochemical (EC) assays for detection of nucleic acids — DNA or RNA — as potential cancer biomarkers. Various ingenious strategies for determination of DNA methylation of gene promoters, circulating tumor DNAs, viral nucleic acids, or short noncoding microRNAs were presented, many of them showing remarkable sensitivities. However, a majority of these assays were not applied into clinical samples from patients, which is crucial should the electrochemistry compete with conventional, routinely used techniques. In this review, we critically evaluate strengths and weaknesses of EC assays that recognized this necessity and successfully determined endogenous DNA or RNA in patient samples with various forms of tumors.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA modifications and cancer · Molecular Biology Techniques and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (54)
Show source-provided references
1. Basic concepts and recent advances in electrochemical analysis of nucleic acids10.1016/j.coelec.2017.09.026 ↗
2. Understanding the factors affecting the analytical performance of sandwich-hybridization genosensors on gold electrodes10.1002/elan.201800049 ↗
3. Electrochemical biosensing in cancer diagnostics and follow-up10.1002/elan.201800193 ↗
4. Current trends and challenges in bioelectrochemistry for non-invasive and early diagnosis10.1016/j.coelec.2018.04.015 ↗
5. Electrochemistry of nucleic acids10.1021/cr200303p ↗
6. Bioelectrochemistry of nucleic acids for early cancer diagnostics - analysis of DNA methylation and detection of microRNAs10.1515/revac-2016-0022 ↗
7. The role of DNA methylation in mammalian epigenetics10.1126/science.1063852 ↗
8. Gene silencing in cancer in association with promoter hypermethylation10.1056/NEJMra023075 ↗
9. DNA methylation in cancer: too much, but also too little10.1038/sj.onc.1205651 ↗
10. Electrochemical sensing of cancer-related global and locus-specific DNA methylation events10.1002/elan.201800004 ↗
11. Current trends in electrochemical sensing and biosensing of DNA methylation10.1016/j.bios.2017.06.004 ↗
12. Electrochemical biosensing strategies for DNA methylation analysis10.1016/j.bios.2017.02.026 ↗
13. Quantification of gene-specific DNA methylation in oesophageal cancer via electrochemistry10.1016/j.aca.2017.04.034 ↗
14. Ultrasensitive homogeneous electrochemical strategy for DNA methyltransferase activity assay based on autonomous exonuclease III-assisted isothermal cycling signal amplification10.1016/j.bios.2015.03.060 ↗
15. Label-free electrochemical detection of human methyltransferase from tumors10.1073/pnas.1417351111 ↗
16. DNA electrochemistry shows DNMT1 methyltransferase hyperactivity in colorectal tumors10.1016/j.chembiol.2015.05.019 ↗
17. Signal amplification of graphene oxide combining with restriction endonuclease for site-specific determination of DNA methylation and assay of methyltransferase activity10.1021/ac301990f ↗
18. Electrochemical assay for the signal-on detection of human DNA methyltransferase activity10.1021/ja4085918 ↗
19. A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension10.1039/c2cc32397d ↗
20. Label-free electrochemical detection of methyltransferase activity and inhibitor screening based on endonuclease HpaII and the deposition of polyaniline10.1016/j.bios.2015.05.066 ↗
21. Highly sensitive electrochemical methyltransferase activity assay10.1021/ac403716g ↗
22. Magnetic bead-based electrochemical assay for determination of DNA methyltransferase activity10.1016/j.electacta.2017.02.104 ↗
23. Curvature-mediated surface accessibility enables ultrasensitive electrochemical human methyltransferase analysis10.1021/acssensors.8b00494 ↗
24. Rapid electrochemical assessment of tumor suppressor gene methylations in raw human serum and tumor cells and tissues using immunomagnetic beads and selective DNA hybridization10.1002/ange.201804339 ↗
25. Versatile electroanalytical bioplatforms for simultaneous determination of cancer-related DNA 5-methyl- and 5-hydroxymethyl-cytosines at global and gene-specific levels in human serum and tissues
26. The role of 5-hydroxymethylcytosine in human cancer10.1007/s00441-014-1896-7 ↗
27. Non-blood circulating tumor DNA detection in cancer10.18632/oncotarget.19942 ↗
28. Circulating tumor DNA: measurement and clinical utility10.1146/annurev-med-041316-085721 ↗
29. Electrochemical genosensing of circulating biomarkers10.3390/s17040866 ↗
30. Circulating tumor DNA and liquid biopsy: opportunities, challenges, and recent advances in detection technologies10.1039/C8LC00100F ↗
31. Liquid biopsies come of age: towards implementation of circulating tumour DNA10.1038/nrc.2017.7 ↗
32. Detection and quantification of mutations in the plasma of patients with colorectal tumors10.1073/pnas.0507904102 ↗
33. Liquid biopsies: genotyping circulating tumor DNA10.1200/JCO.2012.45.2011 ↗
34. Spontaneous release of DNA by human blood lymphocytes as shown in an in vitro system
35. A dual biomarker detection platform for quantitating circulating tumor DNA (ctDNA)10.7150/ntno.22419 ↗
36. Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination–amplification strategy10.1039/C7SC01035D ↗
37. Accurate electrochemistry analysis of circulating methylated DNA from clinical plasma based on paired-end tagging and amplifications10.1021/acs.analchem.7b02572 ↗
38. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples10.1016/j.aca.2018.08.020 ↗
39. Nucleic aptamer modified porous reduced graphene oxide/MoS2 based electrodes for viral detection: application to human papillomavirus (HPV)10.1016/j.snb.2018.02.065 ↗
40. Electrochemical paper-based peptide nucleic acid biosensor for detecting human papillomavirus10.1016/j.aca.2016.11.071 ↗
41. Amplified detection of hepatitis B virus using an electrochemical DNA biosensor on a nanoporous gold platform10.1016/j.bioelechem.2017.06.006 ↗
42. Hydroxyapatite nanoparticles modified graphite electrodes for electrochemical DNA detection10.1002/elan.201700462 ↗
43. Fast electrochemical miRNAs determination in cancer cells and tumor tissues with antibody-functionalized magnetic microcarriers10.1021/acssensors.6b00266 ↗
44. Development of a portable electrochemical loop mediated isothermal amplification (LAMP) device for detection of hepatitis B virus10.1039/C8RA07235C ↗
45. Magnetic bead/capture DNA/glucose-loaded nanoliposomes for amplifying the glucometer signal in the rapid screening of hepatitis C virus RNA10.1007/s00216-018-1055-1 ↗
46. Electrochemical chip-based genomagnetic assay for detection of high-risk human papillomavirus DNA10.1016/j.bios.2016.04.035 ↗
47. Loop-mediated isothermal amplification of DNA10.1093/nar/28.12.e63 ↗
48. microRNA biosensors: opportunities and challenges among conventional and commercially available techniques10.1016/j.bios.2017.08.007 ↗
49. Recent advances in signal amplification strategy based on oligonucleotide and nanomaterials for microRNA detection-a review10.1016/j.bios.2017.08.036 ↗
50. Bioelectrochemistry for miRNA detection10.1016/j.coelec.2017.09.014 ↗
51. Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer10.1111/jcmm.13487 ↗
52. Gold-loaded nanoporous ferric oxide nanocubes for electrocatalytic detection of microRNA at attomolar level10.1016/j.bios.2017.09.027 ↗
53. Multiplexed immunosensing platform coupled to hybridization chain reaction for electrochemical determination of microRNAs in clinical samples
54. Sensitive electrochemical determination of miRNAs based on a sandwich assay onto magnetic microcarriers and hybridization chain reaction amplification10.1016/j.bios.2016.07.003 ↗