Recent advances in peptide-based electrodes for health monitoring: A mini-review

1. Regan B, O’Kennedy R, Eglin D. Advances in Clinical Chemistry. In: Advances in point-of-care testing for cardiovascular diseases. 2021. p. 1–70.10.1016/bs.acc.2020.09.001 ↗

2. Biosensors Market Size & Outlook, 2030 [Internet]. [cited 2026 Jun 23]. Available from: https://www.grandviewresearch.com/horizon/outlook/biosensors-market-size/global

3. Peptide-based biosensors10.1016/j.talanta.2014.12.020 ↗

4. Peptide-based electrochemical biosensing10.1016/j.snb.2021.130232 ↗

5. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes

6. Peptide-Based Plasmon-Enhanced Spectroscopic Immunoassay to Detect Immunity Against Cytomegalovirus10.3390/bios15120817 ↗

7. Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications10.1021/acs.chemrev.1c00044 ↗

8. Overview on peptide-based electrochemical biosensors10.1016/j.ijoes.2023.100395 ↗

9. Electrochemical aptamer-based biosensors as potential tools for clinical diagnostics10.1039/C6AY00499G ↗

10. The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics10.3390/bios11080246 ↗

11. Antibodies and antibody-derived analytical biosensors10.1042/EBC20150002 ↗

12. Guide to Selecting a Biorecognition Element for Biosensors10.1021/acs.bioconjchem.8b00592 ↗

13. Antigen-antibody interactions at the molecular level: adventures in peptide synthesis10.1016/0167-5699(85)90096-9 ↗

14. Peroxidase model electrodes: heme peptide modified electrodes as reagentless sensors for hydrogen peroxide10.1021/ac00015a015 ↗

15. Electrochemical Peptide Biosensor Based on in Situ Silver Deposition for Detection of Prostate Specific Antigen10.1021/acsami.5b01827 ↗

16. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection10.3390/molecules26113200 ↗

17. Context dependence in assembly code for supramolecular peptide materials and systems10.1038/s41578-025-00782-6 ↗

18. An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives10.3390/s18124461 ↗

19. Robust Electrochemical Biosensors Based on Antifouling Peptide Nanoparticles for Protein Quantification in Complex Biofluids10.1021/acssensors.3c02706 ↗

20. Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies10.1021/acs.analchem.3c03127 ↗

21. Highly sensitive electrochemical allergen biosensor based on chitosan nanowhisker-graphene oxide nanohybrid via controllable peptide binder10.1016/j.cej.2026.175085 ↗

22. A robust saliva biosensor for the detection of ANXA2 based on multifunctional peptides immobilized through a strategy biomimicking the dental plaque formation process10.1016/j.snb.2026.139725 ↗

23. Peptide-based electrochemical biosensors utilized for protein detection10.1016/j.bios.2021.113087 ↗

24. A review of the use of genetically engineered enzymes in electrochemical biosensors10.1016/j.semcdb.2009.01.009 ↗

25. Controlling the signal through interfacial design in aptamer-based electrochemical sensors10.1039/D5SD00181A ↗

26. Lyu B, Zhou H, Wang H, Huang Z, Xu Y, Hu Z, et al. Empowering Healthcare with Phage Display Technology. Vol. 11. 2025;11:3228–3248. doi:https://doi.org/10.1021/acsbiomaterials.5c0021810.1021/acsbiomaterials.5c00218 ↗

27. Virtual Screening of Peptide Libraries: The Search for Peptide-Based Therapeutics Using Computational Tools10.3390/ijms25031798 ↗

28. One-shot design of functional protein binders with BindCraft10.1038/s41586-025-09429-6 ↗

29. De novo design of peptide binders to conformationally diverse targets with contrastive language modeling10.1126/sciadv.adr8638 ↗

30. Kong X, Jiao R, Lin H, Guo R, Huang W, Ma WY, et al. Peptide design through binding interface mimicry with PepMimic. 2025. doi:https://doi.org/10.1038/s41551-025-01507-410.1101/2025.01.21.634006 ↗

31. In silico screening of protein-binding peptides with an application to developing peptide inhibitors against antibiotic resistance10.1093/pnasnexus/pgae541 ↗

32. BoltzGen: Toward Universal Binder Design

33. Hu S, Lu Q, Xu Y. Biosensors based on direct electron transfer of protein. In: Electrochemical Sensors, Biosensors and their Biomedical Applications. Academic Press; 2008. p. 531–581. doi:10.1016/B978-0-12-373738-0.X5001-610.1016/B978-012373738-0.50019-2 ↗

34. Affinity peptide-based electrochemical biosensor with 2D-2D nanoarchitecture of nickel–chromium-layered double hydroxide and graphene oxide nanosheets for chirality detection of symmetric dimethylarginine10.1016/j.bios.2024.116871 ↗

35. Bioreceptors as the key components for electrochemical biosensing in medicine10.1016/j.xcrp.2024.101801 ↗

36. A systematic analysis of DMTMM vs EDC/NHS for ligation of amines to Hyaluronan in water10.1016/j.carbpol.2014.02.070 ↗

37. High-sensitivity Ara h1 detection with a praseodymium niobate-carbon nanofiber immunosensor10.1016/j.foodchem.2025.144922 ↗

38. Target-mediated silver deposition-based electrochemical biosensor for highly sensitive detection of human chorionic gonadotropin10.1016/j.bios.2024.116830 ↗

39. Designing an Electrochemical Biosensor Based on Voltammetry for Measurement of Human Chorionic Gonadotropin10.4103/jmss.jmss_64_23 ↗

40. A Robust Biosensor Based on the Pd−S Bond-Immobilized Peptide toward Antifouling Electrochemical Detection of an Alzheimer’s Disease Biomarker in Serum10.1021/acssensors.6c00113 ↗

41. Alake J, Mahlalela MC, Mohite S, Partap S, Gafar MA, Adu DK, et al. Computational, microscale thermophoresis and electrochemical study of a hepta-peptide for a simple, sensitive electrochemical biosensor for PSA. Vol. 206. 2024;206 (111386). doi:10.1016/j.microc.2024.11138610.1016/j.microc.2024.111386 ↗

42. Ren Y, Li J, Wu W, Yu X, Tao M, Han Y, et al. Dual chemical bonding construction of electrochemical peptide sensor based on GDY/MOFs(Fe) composite for ultra-low determination of prostate-specific antigen. Vol. 278. 2024;278 (126459). doi:https://doi.org/10.1016/j.talanta.2024.12645910.1016/j.talanta.2024.126459 ↗

43. Shrink polymer based electrochemical sensor for point-of-care detection of prostate-specific antigen10.1016/j.bios.2023.115193 ↗

44. Ca2+ -Triggered pH-Response Sodium Alginate Hydrogel Precipitation for Amplified Sandwich-Type Impedimetric Immunosensor of Tumor Marker10.1021/acssensors.8b01465 ↗

45. A Robust Nonfouling Biosensor Based on an Engineered Multifunctional Monocyclic Peptide for Electrochemical Detection of Biomarkers in Human Blood10.1021/acs.analchem.5c07842 ↗

46. A Three-Dimensional Electrochemiluminescence Sensor Integrated with Peptide Hydrogel for Detection of H2 O2 Released from Different Subtypes of Breast Cancer Cells10.1021/acs.analchem.4c01625 ↗

47. Prado IC, Carvalho JPRS, Araujo AS, Napoleão-Pêgo P, De-Simone SG. Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue. 2024. doi:https://doi.org/10.1101/2025.11.20.68949410.20944/preprints202411.2364.v1 ↗

48. Highly sensitive and specific graphene/TiO2 impedimetric immunosensor based on plant-derived tetravalent envelope glycoprotein domain III (EDIII) probe antigen for dengue diagnosis10.1016/j.bios.2020.112895 ↗

49. An electrochemical sensor for the rapid detection of zearalenone based on the mimic peptide screened by molecular simulation10.1016/j.foodchem.2024.140364 ↗

50. Development and comparison of electrochemical peptide biosensor based on AuNPs@f-CNFs or AuNPs/rGO-COOH-Atp for detection of zearalenone in cereals10.1016/j.foodchem.2025.147311 ↗

51. Fabrication of screen-printed electrodes: opportunities and challenges10.1007/s10853-020-05499-1 ↗

52. Advanced electrochemical biosensing of pathogens: Harnessing the antimicrobial properties of Ib-M peptides for highly sensitive bacterial detection10.1371/journal.pone.0337227 ↗

53. Modification of polyacrylonitrile-derived carbon nanofibers and bacteriophages on screen-printed electrodes: A portable electrochemical biosensor for rapid detection of Escherichia coli10.1016/j.bioelechem.2022.108229 ↗

54. An Overview of Recent Developments on Electrodes Modified with Bacteriophages10.1002/elan.70026 ↗

55. Phage-based Electrochemical Sensors: A Review10.3390/mi10120855 ↗

56. An electrochemical biosensor with enhanced antifouling properties enabled by peptide self-assembly via robust Pt-S interactions10.1016/j.snb.2024.136321 ↗

57. Antifouling electrochemical biosensors based on mussel-inspired poly(norepinephrine) and functional peptides for the detection of extracellular signal-regulated kinase 2 in serum10.1016/j.snb.2024.136964 ↗

58. Peptide Receptor‐Functionalized AuNP‐Embedded Zwitterionic Biopolymeric Nanohydrogel for Electrochemical Sclerostin Sensing10.1002/advs.74575 ↗

59. A reagent-free, fouling-resistant electrochemical multifunctional peptide sensor for highly sensitive and rapid detection of Alzheimer’s disease biomarkers10.1016/j.mtbio.2025.102081 ↗

60. Design of Dendritic Zwitterionic Oligopeptides with Superior Antifouling Capability for Electrochemical Detection in Diverse Complex Biological Fluids10.1021/acs.analchem.5c04886 ↗

61. A low-fouling electrochemical biosensor based on multifunction branched peptides with antifouling, antibacterial and recognizing sequences for protein detection in saliva10.1016/j.snb.2024.135322 ↗

62. Electrochemical peptide-based biosensor for the detection of the inflammatory disease biomarker, interleukin-1beta10.1016/j.aca.2024.342287 ↗

63. A protease-resistant and anti-fouling electrochemical biosensing interface constructed with dl-serine modified Y-shaped peptides for reliable analysis of aminopeptidase N in human serum10.1016/j.aca.2026.345185 ↗

64. A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds10.1016/j.jinorgbio.2023.112441 ↗