DataVXAdvanced Functional MaterialsPaper
journal article·2025-11Open access

Nanofiber‐Reinforced Self‐Adaptive Hydrogels with Desired Sequential Delivery Capability of Bioactive Factors and Magnesium for Vascularized Osteochondral Regeneration

Xiaolian Niu, Shengzhao Xiao, Kaimin Li, Wei He, Di Huang, Han Yu, Chao Xue, Qiang Cai, Nicholas Dunne, Xiaoming Li

Advanced Functional Materials· Volume 35· Issue 47
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Source abstract

Abstract

Abstract The limited ability of cartilage regeneration, the complexity of joint defects, and the lack of stem cells have plagued the reconstruction of osteochondral defects with tissue engineering scaffolds. This study develops a multifunctional hydrogel for vascularized osteochondral regeneration by incorporating transforming growth factor‐β3 (TGF‐β3)‐loaded Mg‐based nanosheets into nanofiber‐reinforced self‐adaptive hydrogel loaded with platelet‐derived growth factor‐BB (PDGF‐BB). This strategy successfully integrates self‐adaptive and self‐healing properties, reactive oxygen species (ROS) scavenging ability, and adhesion properties within a single scaffold, rendering it suitable for joint repair applications. The β‐sheet‐rich silk nanofibers enhance the mechanical strength of the dynamic hydrogel. Mg‐based nanosheets with high loading efficiency achieve sustainable release of TGF‐β3 and Mg 2+ . The sequential release of PDGF‐BB and TGF‐β3/Mg 2+ from the scaffold stimulates cell migration, tube formation, and differentiation of stem cells. Upon implantation into the osteochondral defect models, the scaffold offers the required stimulus to promote cell recruitment and angiogenesis, and enhance the directed differentiation of stem cells, ultimately achieving satisfactory osteochondral regeneration. Transcriptome analysis in vivo further confirms that the upregulation of relevant genes and signaling pathways is associated with the cell recruitment, angiogenesis, osteogenesis, chondrogenesis, and potential inflammatory regulation, aligning with the findings. These findings suggest that the scaffold provides an optimal 3D microenvironment for osteochondral repair.
Source: crossref · Quality: baseline

Research context

PolymersNanomaterialsBiomaterialsComposites

OpenAlex topics: Bone Tissue Engineering Materials · Electrospun Nanofibers in Biomedical Applications · Connective tissue disorders research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Key Laboratory of Innovation and Transformation of Advanced Medical Devices of Ministry of Industry and Information Technology National Medical Innovation Platform for Industry‐Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering) Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University Beijing 100083 China

Department of Orthodontics Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine College of Stomatology Shanghai Jiao Tong University National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai 200011 China

Department of Biomedical Engineering Research Center for Nano‐biomaterials & Regenerative Medicine College of Biomedical Engineering Taiyuan University of Technology Taiyuan 030024 China

Senior Department of Orthopedics the Fourth Medical Center of PLA General Hospital Beijing 100048 China

Key Laboratory of Advanced Materials of Ministry of Education Tsinghua University Beijing 100084 China

Centre for Medical Engineering Research School of Mechanical and Manufacturing Engineering Dublin City University Stokes Building Collins Avenue Dublin 9 Ireland

Funding

National Natural Science Foundation of China · Natural Science Foundation of Beijing Municipality · Fok Ying Tung Education Foundation

References (70)

Show source-provided references
1. 10.1038/s41413-025-00417-010.1038/s41413-025-00417-0 ↗
2. 10.1038/s41467-024-48579-510.1038/s41467-024-48579-5 ↗
3. 10.1126/scitranslmed.300372010.1126/scitranslmed.3003720 ↗
4. 10.1038/nrrheum.2014.7910.1038/nrrheum.2014.79 ↗
5. 10.1002/EXP.2021001110.1002/EXP.20210011 ↗
6. 10.1002/adma.20160233210.1002/adma.201602332 ↗
7. 10.1016/j.actbio.2021.04.00910.1016/j.actbio.2021.04.009 ↗
8. 10.1016/j.carbpol.2021.11877010.1016/j.carbpol.2021.118770 ↗
9. 10.1002/adfm.20220531510.1002/adfm.202205315 ↗
10. 10.1002/adma.20150427610.1002/adma.201504276 ↗
11. 10.1038/nmat270410.1038/nmat2704 ↗
12. 10.1021/acsami.8b1862610.1021/acsami.8b18626 ↗
13. 10.1016/S0140-6736(10)60668-X10.1016/S0140-6736(10)60668-X ↗
14. 10.1016/j.biomaterials.2015.01.00110.1016/j.biomaterials.2015.01.001 ↗
15. 10.1016/j.actbio.2023.03.04510.1016/j.actbio.2023.03.045 ↗
16. 10.1016/j.bioactmat.2022.06.01110.1016/j.bioactmat.2022.06.011 ↗
17. 10.1038/s41467-023-36408-010.1038/s41467-023-36408-0 ↗
18. 10.1016/j.cej.2021.13042810.1016/j.cej.2021.130428 ↗
19. 10.1038/nm.366810.1038/nm.3668 ↗
20. 10.1089/ten.tea.2015.052410.1089/ten.tea.2015.0524 ↗
21. 10.1016/j.biomaterials.2010.05.01310.1016/j.biomaterials.2010.05.013 ↗
22. 10.1016/j.carbpol.2019.11555110.1016/j.carbpol.2019.115551 ↗
23. 10.1016/j.jconrel.2024.08.01110.1016/j.jconrel.2024.08.011 ↗
24. 10.1039/C7CS00318H10.1039/C7CS00318H ↗
25. 10.1021/cr200434v10.1021/cr200434v ↗
26. 10.1002/adma.20220654510.1002/adma.202206545 ↗
27. 10.1016/j.lfs.2016.01.02910.1016/j.lfs.2016.01.029 ↗
28. 10.1038/s41392-022-01080-110.1038/s41392-022-01080-1 ↗
29. 10.1039/C3PY01190A10.1039/C3PY01190A ↗
30. 10.1038/nature0893610.1038/nature08936 ↗
31. 10.1007/s13238-020-00692-z10.1007/s13238-020-00692-z ↗
32. 10.1021/acsnano.2c0161610.1021/acsnano.2c01616 ↗
33. 10.1021/bm500662z10.1021/bm500662z ↗
34. 10.1016/j.scitotenv.2023.16916010.1016/j.scitotenv.2023.169160 ↗
35. 10.1016/j.actbio.2021.03.05710.1016/j.actbio.2021.03.057 ↗
36. 10.1016/j.biomaterials.2018.08.04410.1016/j.biomaterials.2018.08.044 ↗
37. 10.1016/j.biomaterials.2021.12083810.1016/j.biomaterials.2021.120838 ↗
38. 10.1002/adfm.20200013010.1002/adfm.202000130 ↗
39. 10.1038/nmat448910.1038/nmat4489 ↗
40. 10.1007/s42765-023-00272-w10.1007/s42765-023-00272-w ↗
41. 10.3390/jcm810160210.3390/jcm8101602 ↗
42. 10.1038/s41413-024-00325-910.1038/s41413-024-00325-9 ↗
43. 10.1016/j.molcel.2013.07.01410.1016/j.molcel.2013.07.014 ↗
44. 10.1038/nrc192610.1038/nrc1926 ↗
45. 10.1038/cr.2008.31610.1038/cr.2008.316 ↗
46. 10.1038/nrm229710.1038/nrm2297 ↗
47. 10.1016/j.jconrel.2024.03.05410.1016/j.jconrel.2024.03.054 ↗
48. 10.1016/j.bone.2004.10.01010.1016/j.bone.2004.10.010 ↗
49. 10.1039/C8BM00910D10.1039/C8BM00910D ↗
50. 10.1172/JCI3158110.1172/JCI31581 ↗
51. 10.7150/thno.4542210.7150/thno.45422 ↗
52. 10.3390/polym1323419910.3390/polym13234199 ↗
53. 10.1002/adfm.20230482910.1002/adfm.202304829 ↗
54. Yu Y. · Sci. Adv. · 2021 · 7 · 1550
55. 10.1038/sj.jid.570070110.1038/sj.jid.5700701 ↗
56. 10.1016/j.mtbio.2025.10162210.1016/j.mtbio.2025.101622 ↗
57. 10.1038/s41578-019-0150-z10.1038/s41578-019-0150-z ↗
58. 10.1002/adma.20231025810.1002/adma.202310258 ↗
59. 10.1016/j.biomaterials.2014.07.00610.1016/j.biomaterials.2014.07.006 ↗
60. 10.1016/j.mattod.2021.04.01610.1016/j.mattod.2021.04.016 ↗
61. 10.1016/j.joca.2014.02.88410.1016/j.joca.2014.02.884 ↗
62. 10.4252/wjsc.v16.i2.17610.4252/wjsc.v16.i2.176 ↗
63. 10.1002/adma.20170738910.1002/adma.201707389 ↗
64. 10.1039/C4CC04760E10.1039/C4CC04760E ↗
65. 10.1016/j.eurpolymj.2020.10986110.1016/j.eurpolymj.2020.109861 ↗
66. 10.1080/1465324060085590510.1080/14653240600855905 ↗
67. 10.1002/EXP.2022014910.1002/EXP.20220149 ↗
68. 10.1016/j.actbio.2018.03.04710.1016/j.actbio.2018.03.047 ↗
69. 10.1002/adma.20231255910.1002/adma.202312559 ↗
70. 10.1007/s42765-022-00153-810.1007/s42765-022-00153-8 ↗