DataVXAdvanced Functional MaterialsPaper
journal article·2024-01

3D Assembly of Cryo(Bio)Printed Modular Units for Shelf‐Ready Scalable Tissue Fabrication

Zeyu Luo, Liming Lian, Thiago Stocco, Jie Guo, Xuan Mei, Ling Cai, Syed Muntazir Andrabi, Yajuan Su, Guosheng Tang, Hossein Ravanbakhsh, Wanlu Li, Mian Wang, Xiao Kuang, Carlos Ezio Garciamendez‐Mijares, Di Wang, Zongliang Wang, Junlong Liao, Maobin Xie, Jingwei Xie, Heemin Kang, Anderson Oliveira Lobo, Zongke Zhou, Yu Shrike Zhang

Advanced Functional Materials· Volume 34· Issue 4
Publisher page
Source abstract

Abstract

Abstract The human body relies on modular assembly for realizing its functions. Here the development of a bioinspired cryo(bio)printing‐based method is reported to fabricate shelf‐ready, storable modular scaffolds toward scalable tissue assembly. The mechanism lies in that the cryo(bio)printed modular scaffolds are first assembled into the final hierarchy in their frozen state, which can be subsequently bonded together into an integral piece by contacting each other at the interface during the melting and photocrosslinking processes. This method not only addresses the height limitation associated with the recently developed cryo(bio)printing technology by enabling scalable tissue fabrication through modular assembly, but also allows generating tissue constructs of same or dissimilar materials to fit defects of different scales and shapes, thus providing more precision treatment. Cellular evaluations on the cryobioprinted modular hydrogels validate cell viability, spreading, and differentiation following assembly. The chick ex ovo and rat subcutaneous implantation assays further confirm the potential of direct in vivo assembly using shelf‐ready cryobioprinted modular tissue constructs.
Source: crossref · Quality: baseline

Research context

PolymersBiomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Additive Manufacturing and 3D Printing Technologies · Bone Tissue Engineering Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge MA 02139 USA

Department of Orthopedics West China Hospital/West China School of Medicine Sichuan University Chengdu 610041 P. R. China

Faculty of Medical Sciences Unicamp – State University of Campinas Campinas SP 13083‐877 Brazil

Bioengineering Program, Scientific and Technological Institute Brazil University São Paulo SP 08230‐030 Brazil

Department of Surgery‐Transplant and Mary & Dick Holland Regenerative Medicine Program College of Medicine University of Nebraska Medical Center Omaha NE 68198 USA

Department of Mechanical Engineering McGill University Montreal QC H3A0C3 Canada

Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea

College of Medicine Korea University Seoul 02841 Republic of Korea

Interdisciplinary Laboratory for Advanced Materials BioMatLab Department of Materials Engineering Federal University of Piauí (UFPI) Teresina PI 64049‐550 Brazil

Funding

Brigham Research Institute

References (26)

Show source-provided references
1. 10.1002/adma.20190642310.1002/adma.201906423 ↗
2. 10.1038/s41578-018-0006-y10.1038/s41578-018-0006-y ↗
3. 10.1038/s41551-019-0471-710.1038/s41551-019-0471-7 ↗
4. 10.1002/smll.20180551010.1002/smll.201805510 ↗
5. 10.1002/adma.20210893110.1002/adma.202108931 ↗
6. 10.1016/j.matt.2021.11.02010.1016/j.matt.2021.11.020 ↗
7. B. K. K.Bayles inProceedings of theASME 2012 International Mechanical Engineering Congress and Exposition. Volume 2: Biomedical and Biotechnology ASME Houston Texas November 9–15 2012 pp.615–619.
8. Ellinger I. · 2014
9. 10.1126/science.aaf362710.1126/science.aaf3627 ↗
10. 10.1002/adma.20210211310.1002/adma.202102113 ↗
11. 10.1038/s41563-021-01123-y10.1038/s41563-021-01123-y ↗
12. 10.1038/s41589-021-00934-z10.1038/s41589-021-00934-z ↗
13. 10.1002/adfm.20210777310.1002/adfm.202107773 ↗
14. 10.1002/adma.20200173610.1002/adma.202001736 ↗
15. 10.1002/adfm.20201125210.1002/adfm.202011252 ↗
16. 10.1016/j.actbio.2018.07.03910.1016/j.actbio.2018.07.039 ↗
17. 10.1002/adma.20210893110.1002/adma.202108931 ↗
18. 10.1186/s12891-017-1748-110.1186/s12891-017-1748-1 ↗
19. 10.1016/j.bioactmat.2021.12.01310.1016/j.bioactmat.2021.12.013 ↗
20. 10.3389/fbioe.2022.82523110.3389/fbioe.2022.825231 ↗
21. 10.1016/j.biomaterials.2009.01.04510.1016/j.biomaterials.2009.01.045 ↗
22. 10.1016/j.burns.2022.02.01510.1016/j.burns.2022.02.015 ↗
23. 10.1097/SCS.000000000000701310.1097/SCS.0000000000007013 ↗
24. Osugi I. · Plast. Reconstr. Surg. · 2021 · 9
25. 10.1002/adfm.20180717310.1002/adfm.201807173 ↗
26. 10.1038/s41467-020-14997-410.1038/s41467-020-14997-4 ↗