journal article·2024-08Open access

Egg White Photocrosslinkable Hydrogels as Versatile Bioinks for Advanced Tissue Engineering Applications

Mahboobeh Mahmoodi, Mohammad Ali Darabi, Neda Mohaghegh, Ahmet Erdem, Amir Ahari, Reza Abbasgholizadeh, Maryam Tavafoghi, Paria Mir Hashemian, Vahid Hosseini, Javed Iqbal, Reihaneh Haghniaz, Hossein Montazerian, Jamileh Jahangiry, Fatemeh Nasrolahi, Arshia Mirjafari, Erik Pagan, Mohsen Akbari, Hojae Bae, Johnson V. John, Hossein Heidari, Ali Khademhosseini, Alireza Hassani Najafabadi

Advanced Functional Materials· Volume 34· Issue 32

Abstract

Abstract Three dimensional (3D) bioprinting using photocrosslinkable hydrogels has gained considerable attention due to its versatility in various applications, including tissue engineering and drug delivery. Egg White (EW) is an organic biomaterial with excellent potential in tissue engineering. It provides abundant proteins, along with biocompatibility, bioactivity, adjustable mechanical properties, and intrinsic antiviral and antibacterial features. Here, a photocrosslinkable hydrogel derived from EW is developed through methacryloyl modification, resulting in Egg White methacryloyl (EWMA). Upon exposure to UV light, synthesized EWMA becomes crosslinked, creating hydrogels with remarkable bioactivity. These hydrogels offer adjustable mechanical and physical properties compatible with most current bioprinters. The EWMA hydrogels closely resemble the native extracellular matrix (ECM) due to cell‐binding and matrix metalloproteinase‐responsive motifs inherent in EW. In addition, EWMA promotes cell growth and proliferation in 3D cultures. It facilitates endothelialization when investigated with human umbilical vein endothelial cells (HUVECs), making it an attractive replacement for engineering hemocompatible vascular grafts and biomedical implants. In summary, the EWMA matrix enables the biofabrication of various living constructs. This breakthrough enhances the development of physiologically relevant 3D in vitro models and opens many opportunities in regenerative medicine.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: 3D Printing in Biomedical Research · Electrospun Nanofibers in Biomedical Applications · Hydrogels: synthesis, properties, applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Terasaki Institute for Biomedical Innovation Los Angeles CA 90024 USA

Center for Minimally Invasive Therapeutics (C‐MIT) University of California Los Angeles CA 90095 USA

Department of Bioengineering University of California Los Angeles CA 90095 USA

Department of Biomedical Engineering, Yazd Branch Islamic Azad University Yazd 8915813135 Iran

Joint Reconstruction Research Center Tehran University of Medical Sciences Tehran 1461884513 Iran

Department of Biomedical Engineering, Umuttepe Campus Kocaeli University Kocaeli 41001 Turkey

Department of Biomedical Engineering Boston University Boston MA 02215 USA

Department of Botany Bacha Khan University Charsadda Khyber Pakhtunkhwa 24420 Pakistan

Laboratory for Innovations in MicroEngineering (LiME) Department of Mechanical Engineering University of Victoria Victoria British Columbia V8P 5C2 Canada

Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute Konkuk University Seoul 05029 Republic of Korea

Institute for Materials Discovery University College London London WC1E 6BT UK

Funding

National Institutes of Health · National Institute of Arthritis and Musculoskeletal and Skin Diseases · Natural Sciences and Engineering Research Council of Canada

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