Intelligent Point‐of‐Care Microchip Based on Ternary Hybrids: Incorporating AIEgens and Nanozymes into Metal–Organic Frameworks

Abstract The risk of disease highlights the necessity of point‐of‐care testing. Here, a ternary hybrid (MAP) combining metal–organic frameworks (MOFs), aggregate‐induced emission luminogens, and Pt nanoparticles is introduced. MAP demonstrates a greater fluorescence quantum yield (62.1%) and greater anti‐photobleaching ability than conventional fluorescent MOFs and organic fluorophores, as well as a 2214‐fold and 16‐fold increase in catalytic capacity over those of horseradish peroxidase and Pt nanoparticles, respectively. The MAP exhibited a slight attenuation after 180 days at room temperature in signal intensity. Polydopamine‐coated polystyrene microsphere‐enabled microchips are economical, user‐friendly, and portable. The combination of MAP and microchips allows for determination using the fluorescence/colorimetric mode in different scenarios. Compared with the enzyme‐linked immunosorbent assay, the fluorescence mode exhibited a 41.9‐fold increase in sensitivity, a 94.2% decrease in time, and an 80.7% decrease in cost. The accuracy of clinical samples reached 100%, and the specificity is 96.2%. Chloramphenicol and enrofloxacin serve as small molecule targets, demonstrating the versatility of this strategy.