journal article·2024-06Open access

Injectable and Microporous Microgel Assembly with Sequential Bioactive Factor Release for the Endogenous Repair of Nucleus Pulposus

Huitong Luo, Zetao Wang, Fanglei Yu, Zhiyu Zhou, Jianmin Wang, Dafu Chen, Qi Feng, Xiaodong Cao

Advanced Functional Materials· Volume 34· Issue 25

Abstract

Abstract Harnessing endogenous stem/progenitor cells to enhance extracellular matrix (ECM) deposition in the nucleus pulposus (NP) is considered as a potential treatment for intervertebral disc degeneration (IVDD), but is limited by the lack of suitable scaffolds for cell infiltration and growth, as well as the tailored release of bioactive factors. Herein, an injectable cell‐free microgel assembly (MA‐TNS) with interconnected microporous structure is developed to recruit stem/progenitor cells and induce the differentiation of the recruited cells by releasing stromal cell‐derived factor‐1α (SDF‐1α) and transforming growth factor‐β1 (TGF‐β1) sequentially. TGF‐β1 is initially loaded by the chondroitin sulfate/gelatin nanoparticles. Together with SDF‐1α, these nanoparticles are co‐encapsulated into the photo‐cross‐linked microgels, which are then assembled into a microporous scaffold via dynamic boronate ester bonds. An initial rapid release of SDF‐1α followed by a sustained release of TGF‐β1 lasting over 28 days is achieved. MA‐TNS significantly promotes the recruitment, infiltration, and chondrocyte‐like differentiation of marrow mesenchymal stem cells in vitro. Furthermore, in vivo experiments on rats and cynomolgus monkeys prove that MA‐TNS can inhibit IVDD and recover ECM deposition markedly, thereby enabling long‐term NP reconstruction. The resulting efficacy in rats and nonhuman primates supports that MA‐TNS is a promising scaffold for intervertebral disc repair and regeneration.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Spine and Intervertebral Disc Pathology · Musculoskeletal pain and rehabilitation · Anesthesia and Pain Management

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering School of Materials Science and Engineering South China University of Technology Guangzhou 510006 China

National Engineering Research Center for Tissue Restoration and Reconstruction (NERC‐TRR) South China University of Technology Guangzhou 510006 China

Canwell Medical Co., Ltd Jinhua Zhejiang Province 321016 China

Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve injury Department of Orthopaedic Surgery The Seventh Affiliated Hospital Sun Yat‐sen University Shenzhen 518107 China

Guangdong Provincial Key Laboratory of Orthopedics and Traumatology The First Affiliated Hospital of Sun Yat‐sen University Guangzhou 510080 China

Department of Spinal Surgery Yantaishan Hospital Yantai 264003 China

Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical Materials National Center for Orthopaedics Beijing Research Institute of Traumatology and Orthopaedics Beijing Jishuitan Hospital Beijing 100035 China

Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of Technology Guangzhou 510006 China

Key Laboratory of Biomedical Engineering of Guangdong Province South China University of Technology Guangzhou 510641 China

Funding

National Natural Science Foundation of China · Beijing Municipal Health Commission

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