Synthesis and characterization of Pluronic® grafted chitosan copolymer as a novel injectable biomaterial

Abstract Water-soluble thermosensitive chitosan copolymers were prepared by coupling Pluronic ® onto chitosan using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N -hydroxysuccinimide (NHS) as coupling agents. The physicochemical properties of the resulting copolymers were characterized and they formed thermally reversible hydrogels, which exhibit a lower critical solution temperature (LCST) at 34 °C in aqueous solutions. As a result, chitosan copolymers attest to the usefulness as an injectable material for cell and drug delivery because of their thermally reversible property.