journal article·2025-01Open access

Controlling Alveolar Bone Loss by Hydrogel‐Based Mitigation of Oral Dysbiosis and Bacteria‐Triggered Proinflammatory Immune Response

Xiao Chen, Hanyao Huang, Chenyang Guo, Xuanzhi Zhu, Jiali Chen, Jinzheng Liang, Renjie Yang, Dan Shao, Fangman Chen, Bing Shi, Chao Yang, Kam W. Leong, Lei Zhao

Advanced Functional Materials· Volume 35· Issue 3

Abstract

Abstract Periodontitis is a chronic infection where abnormal host‐microbiota interactions alter the oral microbiome, trigger a proinflammatory immune response, and cause inflammatory alveolar bone loss. While antibiotics are occasionally necessary for treating periodontitis, their use must be carefully managed to prevent the development of drug resistance and oral dysbiosis. Therefore, it's crucial to develop new treatment strategies for periodontitis that reduce antibiotic dependence while effectively controlling the inflammation triggered by bacteria. In this study, a hydrogel is engineered by grafting cationic polyamidoamine dendrimers (PAMAM‐G3) onto the oxidized carboxymethyl cellulose (OCMC) backbone, resulting in an injectable cationic hydrogel (OCMC‐PAMAM‐G3, O‐P). This hydrogel can capture anionic microbial‐associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS) and cell‐free DNA (cfDNA). These findings reveal that using O‐P application circumvents the disruption of the oral mucosa microbiome caused by traditional antibiotics. Additionally, this hydrogel can mitigate inflammatory alveolar bone loss in a ligature‐induced periodontitis mouse model by alleviating the LPS/cfDNA‐TLR4/9 pathway. Moreover, topical administration of O‐P hydrogel has no significant adverse effects on the oral mucosa microbiome while improving the local subgingival microbiome. The study highlights a strategy targeting MAMPs while avoiding antibiotics, as it can mitigate the bacteria‐triggered proinflammatory immune response and potentially preserve oral dysbiosis.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Oral microbiology and periodontitis research · Salivary Gland Disorders and Functions · Oral health in cancer treatment

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu Sichuan 610041 China

Department of Periodontics West China Hospital of Stomatology Sichuan University Chengdu Sichuan 610041 China

Department of Oral and Maxillofacial Surgery West China Hospital of Stomatology Sichuan University Chengdu Sichuan 610041 China

Department of Biomedical Engineering Columbia University New York NY 10027 USA

School of Biomedical Sciences and Engineering Guangzhou International Campus South China University of Technology Guangzhou 511442 China

Department of Orthopedics Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases The Third Affiliated Hospital of Southern Medical University Guangzhou 510630 China

Eastern Clinic West China Hospital of Stomatology Sichuan University Chengdu Sichuan 610041 China

School of Medicine South China University of Technology Guangzhou Guangdong 510006 China

National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou Guangdong 510006 China

Department of Systems Biology Columbia University Medical Center New York NY 10032 USA

Funding

National Natural Science Foundation of China · China Postdoctoral Science Foundation · National Science Foundation

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