Ligand Coupling and Decoupling Modulates Stem Cell Fate

Ramar Thangam; Seong Yeol Kim; Nayeon Kang; Hyunsik Hong; Hyun‐Jeong Lee; Sungkyu Lee; Daun Jeong; Kyong‐Ryol Tag; Kanghyeon Kim; Yangzhi Zhu; Wujin Sun; Han‐Jun Kim; Seung‐Woo Cho; Jae‐Pyoung Ahn; Woo Young Jang; Jong Seung Kim; Ramasamy Paulmurugan; Ali Khademhosseini; Hong‐Kyu Kim; Heemin Kang

doi:10.1002/adfm.202206673

journal article·2023-02Open access

Ligand Coupling and Decoupling Modulates Stem Cell Fate

Ramar Thangam, Seong Yeol Kim, Nayeon Kang, Hyunsik Hong, Hyun‐Jeong Lee, Sungkyu Lee, Daun Jeong, Kyong‐Ryol Tag, Kanghyeon Kim, Yangzhi Zhu, Wujin Sun, Han‐Jun Kim, Seung‐Woo Cho, Jae‐Pyoung Ahn, Woo Young Jang, Jong Seung Kim, Ramasamy Paulmurugan, Ali Khademhosseini, Hong‐Kyu Kim, Heemin Kang

Advanced Functional Materials· Volume 33· Issue 8

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Source abstract

Abstract

Abstract In natural microenvironment, various proteins containing adhesive ligands in fibrous and non‐fibrous structures dynamically couple and decouple to regulate stem cell fate. Herein, materials presenting movably couplable ligands are developed by grafting liganded gold nanoparticles (AuNPs) to a substrate followed by flexibly grafting liganded movable linear nanomaterials (MLNs) to the substrate via a long bendable linker, thereby creating a space between the MLNs and the AuNPs in the decoupled state. Magnetic control of the MLNs decreases this space via the bending of the linker to couple the MLNs to the AuNPs. Remote control of ligand coupling stimulates integrin recruitment to the coupled ligands, thereby non‐toxically facilitating the focal adhesion, mechanosensing, and potential differentiation of stem cells, which is suppressed by ligand decoupling. Versatile tuning of size, aspect ratio, distributions, and ligands of the MLNs can help to decipher dynamic ligand‐coupling‐dependent stem cell fate to advance regenerative therapies.

Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Cellular Mechanics and Interactions · 3D Printing in Biomedical Research · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea

Advanced Analysis Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of Korea

Department of Orthopedic Surgery Korea University Anam Hospital Seoul 02841 Republic of Korea

Terasaki Institute for Biomedical Innovation Los Angeles CA 90064 USA

Department of Biological Systems Engineering Virginia Tech Blacksburg VA 24061 USA

Department of Biotechnology Yonsei University Seoul 03722 Republic of Korea

Center for Nanomedicine Institute for Basic Science (IBS) Seoul 03722 Republic of Korea

Department of Chemistry Korea University Seoul 02841 Republic of Korea

Department of Radiology Molecular Imaging Program at Stanford Stanford University School of Medicine Stanford University Palo Alto CA 94304 USA

Department of Radiology Canary Center at Stanford for Cancer Early Detection Stanford University School of Medicine Stanford University Palo Alto CA 94304 USA

Department of Biomicrosystem Technology Korea University Seoul 02841 Republic of Korea

Funding

National Research Foundation of Korea

References (82)

Show source-provided references

1. 10.1038/nrm390210.1038/nrm3902 ↗

2. 10.1016/j.ydbio.2009.10.02610.1016/j.ydbio.2009.10.026 ↗

3. 10.1038/s41563-019-0504-310.1038/s41563-019-0504-3 ↗

4. 10.1002/adfm.20200622710.1002/adfm.202006227 ↗

5. 10.1002/adfm.20201125210.1002/adfm.202011252 ↗

6. 10.1002/adfm.20210084810.1002/adfm.202100848 ↗

7. 10.1002/ijc.291020010210.1002/ijc.2910200102 ↗

8. 10.1016/j.chempr.2019.05.01110.1016/j.chempr.2019.05.011 ↗

9. 10.1016/j.ajpath.2011.04.01910.1016/j.ajpath.2011.04.019 ↗

10. 10.1002/mus.2209410.1002/mus.22094 ↗

11. 10.1371/journal.pone.012767510.1371/journal.pone.0127675 ↗

12. 10.1002/adfm.20210983310.1002/adfm.202109833 ↗

13. 10.1073/pnas.98.3.104210.1073/pnas.98.3.1042 ↗

14. 10.1038/nrm.2017.10810.1038/nrm.2017.108 ↗

15. 10.1038/ncomms902610.1038/ncomms9026 ↗

16. 10.1038/s41578-020-0209-x10.1038/s41578-020-0209-x ↗

17. 10.1021/bm100357t10.1021/bm100357t ↗

18. 10.1016/j.jmhi.2012.09.00210.1016/j.jmhi.2012.09.002 ↗

19. 10.1002/adma.20210838910.1002/adma.202108389 ↗

20. 10.1002/adma.20200791010.1002/adma.202007910 ↗

21. 10.1002/adma.20210111010.1002/adma.202101110 ↗

22. 10.1002/adma.20200417210.1002/adma.202004172 ↗

23. 10.1002/adfm.20211100310.1002/adfm.202111003 ↗

24. 10.1073/pnas.211096111810.1073/pnas.2110961118 ↗

25. 10.1038/natrevmats.2015.1210.1038/natrevmats.2015.12 ↗

26. 10.1038/s41570-018-012010.1038/s41570-018-0120 ↗

27. 10.1002/VIW.2020002910.1002/VIW.20200029 ↗

28. 10.1016/j.addr.2021.11395410.1016/j.addr.2021.113954 ↗

29. 10.1002/smll.20210289210.1002/smll.202102892 ↗

30. 10.1002/adfm.20210126210.1002/adfm.202101262 ↗

31. 10.1002/adfm.20210598910.1002/adfm.202105989 ↗

32. 10.1002/adma.20210576510.1002/adma.202105765 ↗

33. 10.1016/j.actbio.2021.03.02610.1016/j.actbio.2021.03.026 ↗

34. 10.1038/nmat415710.1038/nmat4157 ↗

35. 10.1039/C8CS00053K10.1039/C8CS00053K ↗

36. 10.1002/adma.20220549810.1002/adma.202205498 ↗

37. 10.1021/ja412364m10.1021/ja412364m ↗

38. 10.1002/adfm.20210744710.1002/adfm.202107447 ↗

39. 10.1016/j.ejcb.2005.09.01110.1016/j.ejcb.2005.09.011 ↗

40. 10.1529/biophysj.106.08973010.1529/biophysj.106.089730 ↗

41. 10.1021/nl104079r10.1021/nl104079r ↗

42. 10.1021/acs.nanolett.0c0265510.1021/acs.nanolett.0c02655 ↗

43. 10.1021/nl104378f10.1021/nl104378f ↗

44. 10.1126/science.276.5317.142510.1126/science.276.5317.1425 ↗

45. 10.1021/nl504986210.1021/nl5049862 ↗

46. 10.1242/jcs.115.7.142310.1242/jcs.115.7.1423 ↗

47. 10.1021/nl803548b10.1021/nl803548b ↗

48. 10.1002/adma.20200430010.1002/adma.202004300 ↗

49. 10.1038/s41563-019-0460-y10.1038/s41563-019-0460-y ↗

50. 10.1038/s41563-020-00896-y10.1038/s41563-020-00896-y ↗

51. 10.1038/s41467-020-17380-510.1038/s41467-020-17380-5 ↗

52. 10.1371/journal.pone.014806610.1371/journal.pone.0148066 ↗

53. 10.1002/adma.20211034010.1002/adma.202110340 ↗

54. 10.1021/jacs.1c0886110.1021/jacs.1c08861 ↗

55. 10.1002/adfm.20210340910.1002/adfm.202103409 ↗

56. 10.1002/adma.20190386210.1002/adma.201903862 ↗

57. 10.1002/adfm.20211120810.1002/adfm.202111208 ↗

58. 10.1002/adfm.20210404610.1002/adfm.202104046 ↗

59. 10.1002/adfm.20210987110.1002/adfm.202109871 ↗

60. 10.1002/adfm.20220082810.1002/adfm.202200828 ↗

61. 10.1021/acsnano.7b0285710.1021/acsnano.7b02857 ↗

62. 10.1002/adma.20200835310.1002/adma.202008353 ↗

63. 10.1002/adma.20210546010.1002/adma.202105460 ↗

64. 10.1038/nmat333910.1038/nmat3339 ↗

65. 10.1038/srep3433410.1038/srep34334 ↗

66. 10.1002/adfm.20200948910.1002/adfm.202009489 ↗

67. 10.1002/adfm.20210306310.1002/adfm.202103063 ↗

68. 10.1002/adfm.20201117110.1002/adfm.202011171 ↗

69. 10.1002/adfm.20220079110.1002/adfm.202200791 ↗

70. 10.1039/b711490g10.1039/b711490g ↗

71. 10.1038/s41563-017-0014-010.1038/s41563-017-0014-0 ↗

72. 10.1002/adfm.20201062610.1002/adfm.202010626 ↗

73. 10.1021/acsami.1c2260310.1021/acsami.1c22603 ↗

74. 10.1021/acs.nanolett.6b0495810.1021/acs.nanolett.6b04958 ↗

75. 10.1002/adma.20200256610.1002/adma.202002566 ↗

76. 10.1016/j.biomaterials.2020.12054310.1016/j.biomaterials.2020.120543 ↗

77. 10.1002/adma.20200698610.1002/adma.202006986 ↗

78. 10.1002/adfm.20200869810.1002/adfm.202008698 ↗

79. 10.1007/s11060-010-0389-010.1007/s11060-010-0389-0 ↗

80. 10.1002/adfm.20200943210.1002/adfm.202009432 ↗

81. 10.1002/adfm.20200966310.1002/adfm.202009663 ↗

82. 10.1038/s41467-019-09733-610.1038/s41467-019-09733-6 ↗