Reconstruction of Muscle Fascicle‐Like Tissues by Anisotropic 3D Patterning
Source abstract
Abstract
Abstract Tissue engineering of skeletal muscle has been proposed as a potential regenerative treatment for extensive muscle damage. In this regard, the highly organized structure of skeletal muscles makes the alignment of cells especially indispensable in muscle tissue engineering. However, achieving the desired alignment continues to prove challenging, particularly in 3D engineered tissue constructs. In this study, a biomimetic approach for the generation of functional skeletal muscle fascicle‐like tissues by recapitulating 3D muscle‐like cellular and extracellular organization, is demonstrated. Anisotropic 3D alignment of muscle extracellular matrix (MEM) nanofibrils capable of providing a pro‐myogenic microenvironment by regulating the kinetics of fibrillogenesis in a stretchable elastomeric chip, is achieved. Reprogrammed muscle progenitor cells develop myofibers along the aligned MEM nanofibrils in a 3D configuration, culminating in the structural and functional maturation of skeletal muscle. The resultant 3D muscle fascicle‐like constructs support de novo muscle regeneration and induce functional restoration of injured muscles in animal models inflicted with volumetric muscle loss and congenital muscular dystrophy. This study not only highlights the fundamental roles of the muscle–mimetic structural guidance cues for 3D muscle tissue engineering, but also unveils the clinical potential of artificial muscle constructs in regenerative medicine.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Tissue Engineering and Regenerative Medicine · Electrospun Nanofibers in Biomedical Applications · Muscle Physiology and Disorders
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biotechnology Yonsei University Seoul 03722 Republic of Korea
Brain Science Institute Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of Korea
Division of Bio‐Medical Science and Technology KIST School Korea University of Science and Technology (UST) Seoul 02792 Republic of Korea
SK Biopharmaceuticals Co. Ltd. Seongnam 13494 Republic of Korea
Department and Research Institute of Rehabilitation Medicine Yonsei University College of Medicine Seoul 03722 Republic of Korea
Department of Chemical Engineering Soongsil University Seoul 06978 Republic of Korea
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Dankook University Cheonan 31116 Republic of Korea
Department of Chemical and Biochemical Engineering Dongguk University Seoul 04620 Republic of Korea
Brain Korea 21 PLUS Project for Medical Science Yonsei University College of Medicine Seoul 03722 Republic of Korea
KU‐KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Republic of Korea
Center for Nanomedicine Institute for Basic Science (IBS) Seoul 03722 Republic of Korea
Graduate Program of Nano Biomedical Engineering (NanoBME) Advanced Science Institute Yonsei University Seoul 03722 Republic of Korea
Funding
National Research Foundation of Korea · Institute for Basic Science
References (98)
Show source-provided references
1. 10.5435/00124635-201102001-0000710.5435/00124635-201102001-00007 ↗
2. 10.1016/S0140-6736(12)61897-210.1016/S0140-6736(12)61897-2 ↗
3. 10.1038/ncomms1561310.1038/ncomms15613 ↗
4. 10.1038/s41467-017-02636-410.1038/s41467-017-02636-4 ↗
5. 10.1038/s41551-018-0290-210.1038/s41551-018-0290-2 ↗
6. 10.1002/mus.2209410.1002/mus.22094 ↗
7. 10.1016/j.biomaterials.2008.04.02810.1016/j.biomaterials.2008.04.028 ↗
8. 10.1021/acs.nanolett.6b0013110.1021/acs.nanolett.6b00131 ↗
9. 10.1016/j.biomaterials.2006.10.01010.1016/j.biomaterials.2006.10.010 ↗
10. 10.1088/1758-5090/aa585e10.1088/1758-5090/aa585e ↗
11. 10.1038/s41467-018-07823-510.1038/s41467-018-07823-5 ↗
12. 10.1002/adma.20150391610.1002/adma.201503916 ↗
13. 10.1007/s10544-006-6380-z10.1007/s10544-006-6380-z ↗
14. 10.1242/jcs.0260510.1242/jcs.02605 ↗
15. 10.1021/am508292t10.1021/am508292t ↗
16. 10.1016/j.biomaterials.2013.12.08110.1016/j.biomaterials.2013.12.081 ↗
17. 10.1038/natrevmats.2018.1610.1038/natrevmats.2018.16 ↗
18. 10.1038/nbt.341310.1038/nbt.3413 ↗
19. 10.1039/C8SM02605J10.1039/C8SM02605J ↗
20. 10.1038/ncomms1434610.1038/ncomms14346 ↗
21. 10.1089/ten.tea.2009.082610.1089/ten.tea.2009.0826 ↗
22. 10.1016/j.biomaterials.2011.07.01610.1016/j.biomaterials.2011.07.016 ↗
23. 10.1016/j.biomaterials.2011.12.05510.1016/j.biomaterials.2011.12.055 ↗
24. 10.1016/j.biomaterials.2012.12.04810.1016/j.biomaterials.2012.12.048 ↗
25. 10.1089/ten.tea.2009.022610.1089/ten.tea.2009.0226 ↗
26. 10.1007/s00383-002-0849-510.1007/s00383-002-0849-5 ↗
27. 10.1016/j.biomaterials.2004.07.03510.1016/j.biomaterials.2004.07.035 ↗
28. 10.1038/s41598-018-26371-y10.1038/s41598-018-26371-y ↗
29. 10.1021/bm401503910.1021/bm4015039 ↗
30. 10.1002/adfm.20180195410.1002/adfm.201801954 ↗
31. 10.1021/acsami.9b0324210.1021/acsami.9b03242 ↗
32. 10.1038/s41598-017-08232-210.1038/s41598-017-08232-2 ↗
33. 10.1016/j.stemcr.2018.04.00910.1016/j.stemcr.2018.04.009 ↗
34. N.Choi Ph.D. Cornell University 2010 04.
35. 10.1073/pnas.172261911510.1073/pnas.1722619115 ↗
36. 10.1371/journal.pone.013621710.1371/journal.pone.0136217 ↗
37. 10.1074/mcp.M111.01464710.1074/mcp.M111.014647 ↗
38. 10.1126/science.126041910.1126/science.1260419 ↗
39. 10.1186/s12864-015-1686-y10.1186/s12864-015-1686-y ↗
40. 10.1002/jcp.2137110.1002/jcp.21371 ↗
41. 10.1016/j.jprot.2012.09.00810.1016/j.jprot.2012.09.008 ↗
42. 10.1038/ncomms296410.1038/ncomms2964 ↗
43. 10.1016/j.matbio.2013.03.00710.1016/j.matbio.2013.03.007 ↗
44. 10.1016/j.bbrc.2012.01.09110.1016/j.bbrc.2012.01.091 ↗
45. 10.1371/journal.pone.007202310.1371/journal.pone.0072023 ↗
46. 10.1074/jbc.R20002720010.1074/jbc.R200027200 ↗
47. 10.1074/jbc.R20002220010.1074/jbc.R200022200 ↗
48. 10.1002/dvdy.2028010.1002/dvdy.20280 ↗
49. 10.1016/S1534-5807(03)00118-710.1016/S1534-5807(03)00118-7 ↗
50. 10.1038/nm.411610.1038/nm.4116 ↗
51. 10.1093/hmg/ddg04710.1093/hmg/ddg047 ↗
52. 10.1242/jcs.0123410.1242/jcs.01234 ↗
53. 10.1083/jcb.143.3.84910.1083/jcb.143.3.849 ↗
54. 10.1016/j.actbio.2016.04.04610.1016/j.actbio.2016.04.046 ↗
55. 10.1083/jcb.138.6.132310.1083/jcb.138.6.1323 ↗
56. 10.1083/jcb.20020203410.1083/jcb.200202034 ↗
57. 10.1039/c0jm01841d10.1039/c0jm01841d ↗
58. 10.1016/j.biomaterials.2012.06.05710.1016/j.biomaterials.2012.06.057 ↗
59. 10.1021/acsnano.5b0364410.1021/acsnano.5b03644 ↗
60. 10.1091/mbc.e17-01-004610.1091/mbc.e17-01-0046 ↗
61. 10.1186/2044-5040-1-2910.1186/2044-5040-1-29 ↗
62. 10.1091/mbc.e09-02-017510.1091/mbc.e09-02-0175 ↗
63. 10.1074/jbc.C60031520010.1074/jbc.C600315200 ↗
64. 10.1016/j.bbrc.2015.07.01810.1016/j.bbrc.2015.07.018 ↗
65. 10.1083/jcb.20070717510.1083/jcb.200707175 ↗
66. 10.1186/2044-5040-1-410.1186/2044-5040-1-4 ↗
67. 10.1016/j.bbadis.2011.01.00210.1016/j.bbadis.2011.01.002 ↗
68. 10.1016/j.yexcr.2010.05.00810.1016/j.yexcr.2010.05.008 ↗
69. 10.1016/j.cell.2005.10.02810.1016/j.cell.2005.10.028 ↗
70. 10.1371/journal.pgen.100269110.1371/journal.pgen.1002691 ↗
71. 10.1002/jcp.2404210.1002/jcp.24042 ↗
72. 10.1242/jcs.115.10.204110.1242/jcs.115.10.2041 ↗
73. 10.3389/fphys.2016.0004110.3389/fphys.2016.00041 ↗
74. 10.1096/fj.201601090R10.1096/fj.201601090R ↗
75. Wang C. · Am. J. Cancer Res. · 2015 · 6 · 27
76. 10.1038/nature1013710.1038/nature10137 ↗
77. 10.1016/j.gde.2015.03.00610.1016/j.gde.2015.03.006 ↗
78. 10.1016/j.bbrc.2010.02.03410.1016/j.bbrc.2010.02.034 ↗
79. 10.1242/jcs.10954610.1242/jcs.109546 ↗
80. 10.1002/adfm.20190386310.1002/adfm.201903863 ↗
81. 10.1111/cts.1251910.1111/cts.12519 ↗
82. 10.1126/science.aad927210.1126/science.aad9272 ↗
83. 10.1007/s00441-003-0757-610.1007/s00441-003-0757-6 ↗
84. 10.1242/dev.03871110.1242/dev.038711 ↗
85. 10.1016/j.biomaterials.2010.05.01910.1016/j.biomaterials.2010.05.019 ↗
86. 10.1016/j.actbio.2017.11.04610.1016/j.actbio.2017.11.046 ↗
87. 10.1089/ten.tea.2016.034010.1089/ten.tea.2016.0340 ↗
88. 10.1038/s41467-019-12545-310.1038/s41467-019-12545-3 ↗
89. 10.1016/j.biomaterials.2015.07.04010.1016/j.biomaterials.2015.07.040 ↗
90. 10.1073/pnas.180830311610.1073/pnas.1808303116 ↗
91. 10.1016/j.stem.2018.06.01010.1016/j.stem.2018.06.010 ↗
92. 10.1038/s41598-017-03703-y10.1038/s41598-017-03703-y ↗
93. 10.1038/s41420-018-0027-810.1038/s41420-018-0027-8 ↗
94. 10.1101/cshperspect.a00602310.1101/cshperspect.a006023 ↗
95. 10.1016/j.actbio.2018.12.00810.1016/j.actbio.2018.12.008 ↗
96. 10.1021/acsami.7b1058310.1021/acsami.7b10583 ↗
97. 10.1016/j.biomaterials.2015.08.04210.1016/j.biomaterials.2015.08.042 ↗
98. 10.1186/2044-5040-1-2110.1186/2044-5040-1-21 ↗