journal article·2023-04Open access

Modulation of Macrophages by In Situ Ligand Bridging

Seong Yeol Kim, Ramar Thangam, Nayeon Kang, Hyunsik Hong, Chowon Kim, Sungkyu Lee, Subin Son, Hyun‐Jeong Lee, Kyong‐Ryol Tag, Sunhong Min, Daun Jeong, Jangsun Hwang, Kanghyeon Kim, Dahee Kim, Yuri Kim, Jinmyoung Joo, Bong Hoon Kim, Yangzhi Zhu, Sung‐Gyu Park, Hyun‐Cheol Song, Wujin Sun, Jae‐Pyoung Ahn, Woo Young Jang, Ramasamy Paulmurugan, Hong‐Kyu Kim, Jong Seung Kim, Heemin Kang

Advanced Functional Materials· Volume 33· Issue 16

Abstract

Abstract Extracellular matrix (ECM) proteins containing cell‐attachable Arg‐Gly‐Asp (RGD) sequences exhibit variable bridging and non‐bridging in fibronectin‐collagen and laminin‐collagen complexes that can regulate inflammation, tissue repair, and wound healing. In this study, linking molecule‐mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre‐decorated with gold nanospheres (GNSs) is performed, thereby yielding RGD‐coated MNCs (RGD‐MNCs) over RGD‐coated GNSs (RGD‐GNSs) in a non‐bridging state. The RGD‐MNCs are drawn closer to the RGD‐GNSs via magnetic field‐mediated compression of the linking molecules to establish the bridging between them. Relative proportion of the RGD‐MNCs to the RGD‐GNSs is optimized to yield effective remote stimulation of integrin binding to variably bridged RGDs similar to that of invariably bridged RGDs used as a control group. Remote manipulation of the RGD bridging facilitates the attachment structure assembly of macrophages that leads to pro‐healing/anti‐inflammatory phenotype acquisition. In contrast, the non‐bridged RGDs inhibited macrophage attachment that acquired pro‐inflammatory phenotypes. The use of various nanomaterials in constructing heterogeneous RGD‐coated materials can further offer various modes in remote switching of RGD bridging and non‐bridging to understand dynamic integrin‐mediated modulation of macrophages that regulate immunomodulatory responses, such as foreign body responses, tissue repair, and wound healing.
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Research context

OpenAlex topics: Cell Adhesion Molecules Research · Polymer Surface Interaction Studies · Cellular Mechanics and Interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Materials Science and Engineering Korea University Seoul 02841 Republic of Korea

Department of Chemistry Korea University Seoul 02841 Republic of Korea

Advanced Analysis Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of Korea

Department of Orthopedic Surgery Korea University Anam Hospital Seoul 02841 Republic of Korea

Department of Biomedical Engineering Ulsan National Institute of Science and Technology (UNIST) Ulsan 44919 Republic of Korea

Daegu Gyeongbuk Institute of Science and Technology (DGIST) Department of Robotics and Mechatronics Engineering Daegu 42988 Republic of Korea

Terasaki Institute for Biomedical Innovation Los Angeles CA 90064 USA

Department of Nano‐Bio Convergence Korea Institute of Materials Science (KIMS) Changwon Gyeongnam 51508 Republic of Korea

Electronic Materials Research Center Korea Institute of Science and Technology (KIST) Seoul 02792 Republic of Korea

KIST‐SKKU Carbon‐Neutral Research Center Sungkyunkwan University (SKKU) Suwon 16419 Republic of Korea

Department of Biological Systems Engineering Virginia Tech Blacksburg VA 24061 USA

Department of Radiology, Molecular Imaging Program at Stanford Stanford University School of Medicine Stanford University Palo Alto CA 94304 USA

Department of Radiology, Canary Center at Stanford for Cancer Early Detection Stanford University School of Medicine Stanford University Palo Alto CA 94304 USA

College of Medicine Korea University Seoul 02841 Republic of Korea

Funding

National Research Foundation of Korea

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