DataVXAdvanced Functional MaterialsPaper
journal article·2022-02Open access

Ultra‐Sharp Nanowire Arrays Natively Permeate, Record, and Stimulate Intracellular Activity in Neuronal and Cardiac Networks

Ren Liu, Jihwan Lee, Youngbin Tchoe, Deborah Pre, Andrew M. Bourhis, Agnieszka D'Antonio‐Chronowska, Gaelle Robin, Sang Heon Lee, Yun Goo Ro, Ritwik Vatsyayan, Karen J. Tonsfeldt, Lorraine A. Hossain, M. Lisa Phipps, Jinkyoung Yoo, John Nogan, Jennifer S. Martinez, Kelly A. Frazer, Anne G. Bang, Shadi A. Dayeh

Advanced Functional Materials· Volume 32· Issue 8
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Abstract

Abstract Intracellular access with high spatiotemporal resolution can enhance the understanding of how neurons or cardiomyocytes regulate and orchestrate network activity and how this activity can be affected with pharmacology or other interventional modalities. Nanoscale devices often employ electroporation to transiently permeate the cell membrane and record intracellular potentials, which tend to decrease rapidly with time. Here, one reports innovative scalable, vertical, ultrasharp nanowire arrays that are individually addressable to enable long‐term, native recordings of intracellular potentials. One reports electrophysiological recordings that are indicative of intracellular access from 3D tissue‐like networks of neurons and cardiomyocytes across recording days and that do not decrease to extracellular amplitudes for the duration of the recording of several minutes. The findings are validated with cross‐sectional microscopy, pharmacology, and electrical interventions. The experiments and simulations demonstrate that the individual electrical addressability of nanowires is necessary for high‐fidelity intracellular electrophysiological recordings. This study advances the understanding of and control over high‐quality multichannel intracellular recordings and paves the way toward predictive, high‐throughput, and low‐cost electrophysiological drug screening platforms.
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Research context

Nanomaterials

OpenAlex topics: Neuroscience and Neural Engineering · Advanced Memory and Neural Computing · Neural dynamics and brain function

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Integrated Electronics and Biointerfaces Laboratory Department of Electrical and Computer Engineering University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Conrad Prebys Center for Chemical Genomics Sanford Burnham Prebys Medical Discovery Institute 10901 North Torrey Pines Road La Jolla CA 92037 USA

Department of Pediatrics University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Center for Reproductive Science and Medicine Department of Obstetrics Gynecology, and Reproductive Sciences University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Center for Integrated Nanotechnologies Los Alamos National Laboratory Los Alamos NM 87545 USA

Center for Integrated Nanotechnologies Sandia National Laboratories Albuquerque NM 87185 USA

Center for Materials Interfaces in Research and Applications and Department of Applied Physics and Materials Science Northern Arizona University 624 S. Knoles Dr. Flagstaff Flagstaff AZ 86011 USA

Funding

National Science Foundation · National Institutes of Health · U.S. Department of Energy · Office of Science · Sandia National Laboratories

References (59)

Show source-provided references
1. 10.1152/jn.2001.85.5.196910.1152/jn.2001.85.5.1969 ↗
2. 10.1007/BF0058424710.1007/BF00584247 ↗
3. 10.1126/science.287.5451.29510.1126/science.287.5451.295 ↗
4. 10.1016/S0306-4522(01)00167-110.1016/S0306-4522(01)00167-1 ↗
5. 10.1038/nmeth.142010.1038/nmeth.1420 ↗
6. 10.1038/nnano.2011.24910.1038/nnano.2011.249 ↗
7. 10.3389/fnins.2018.0021210.3389/fnins.2018.00212 ↗
8. 10.1038/nnano.2012.810.1038/nnano.2012.8 ↗
9. 10.1038/nnano.2011.22310.1038/nnano.2011.223 ↗
10. 10.1038/s41551-019-0455-710.1038/s41551-019-0455-7 ↗
11. 10.1021/acs.nanolett.9b0220910.1021/acs.nanolett.9b02209 ↗
12. 10.1038/s41378-020-0178-710.1038/s41378-020-0178-7 ↗
13. 10.1021/acs.nanolett.7b0152310.1021/acs.nanolett.7b01523 ↗
14. 10.1126/sciadv.abd517510.1126/sciadv.abd5175 ↗
15. Lin Z. C. · Microsyst. Nanoeng. · 2017 · 3 · 1
16. 10.1038/nnano.2013.27310.1038/nnano.2013.273 ↗
17. 10.1038/s41565-019-0478-y10.1038/s41565-019-0478-y ↗
18. 10.1038/nrn338310.1038/nrn3383 ↗
19. 10.1021/nl403201a10.1021/nl403201a ↗
20. 10.1021/ja071456k10.1021/ja071456k ↗
21. 10.1016/j.biomaterials.2012.10.04210.1016/j.biomaterials.2012.10.042 ↗
22. 10.1021/nl303163y10.1021/nl303163y ↗
23. 10.1016/j.biomaterials.2014.11.05110.1016/j.biomaterials.2014.11.051 ↗
24. 10.1126/science.112864010.1126/science.1128640 ↗
25. 10.1021/nl100260810.1021/nl1002608 ↗
26. 10.1021/acs.nanolett.6b0475210.1021/acs.nanolett.6b04752 ↗
27. 10.1021/acs.accounts.7b0059410.1021/acs.accounts.7b00594 ↗
28. 10.1021/acsami.8b0821810.1021/acsami.8b08218 ↗
29. 10.3389/fnins.2020.0005510.3389/fnins.2020.00055 ↗
30. 10.1021/acs.nanolett.9b0229610.1021/acs.nanolett.9b02296 ↗
31. Hossain L. · Proc. SPIE · 2021 · 11629 · 1162929
32. 10.1088/1741-2552/abbd2210.1088/1741-2552/abbd22 ↗
33. 10.1371/journal.pcbi.100676910.1371/journal.pcbi.1006769 ↗
34. 10.1093/cercor/bhab04010.1093/cercor/bhab040 ↗
35. 10.1016/j.clinph.2021.06.02410.1016/j.clinph.2021.06.024 ↗
36. 10.1016/j.neuroimage.2018.04.02710.1016/j.neuroimage.2018.04.027 ↗
37. 10.1002/adfm.20170023210.1002/adfm.201700232 ↗
38. 10.1016/j.nano.2005.03.00710.1016/j.nano.2005.03.007 ↗
39. 10.1021/am402070k10.1021/am402070k ↗
40. 10.1021/la701704910.1021/la7017049 ↗
41. 10.1371/journal.pone.018727610.1371/journal.pone.0187276 ↗
42. 10.1038/s41598-019-40128-110.1038/s41598-019-40128-1 ↗
43. 10.3389/fnins.2018.0063010.3389/fnins.2018.00630 ↗
44. 10.1111/j.1749-6632.2009.04015.x10.1111/j.1749-6632.2009.04015.x ↗
45. 10.1523/ENEURO.0153-16.201710.1523/ENEURO.0153-16.2017 ↗
46. Tanskanen J. M. · 2016 · 66210.1109/EUSIPCO.2016.7760331 ↗
47. Tanskanen J. M. · 2015 · 1016
48. J. M.Tanskanen F. E.Kapucu I.Vornanen K.Lenk J.Hyttinen
49. 10.1016/j.stemcr.2019.09.01110.1016/j.stemcr.2019.09.011 ↗
50. 10.1371/journal.pone.004028810.1371/journal.pone.0040288 ↗
51. D'Antonio‐Chronowska A. · Bio‐Protoc. · 2020 · 10 · e3755
52. Ma J. · Am. J. Physiol. · 2011 · 301 · 599
53. 10.1016/j.stem.2019.02.01510.1016/j.stem.2019.02.015 ↗
54. 10.1098/rsif.2009.008710.1098/rsif.2009.0087 ↗
55. Rajan D. K. · 2017 · 470
56. 10.1038/s42003-019-0393-710.1038/s42003-019-0393-7 ↗
57. Wang J. · 2020 · 1
58. 10.1016/j.jsamd.2020.07.00310.1016/j.jsamd.2020.07.003 ↗
59. 10.1021/acs.nanolett.6b0002010.1021/acs.nanolett.6b00020 ↗