DataVXAdvanced Functional MaterialsPaper
journal article·2023-11Open access

Molecular Engineering of NIR‐II AIE Luminogen Excited at 1700 nm for Ultradeep Intravital Brain Two‐Photon Fluorescence Imaging

Dan Li, Xiangquan Deng, Zhourui Xu, Deliang Wang, Gaixia Xu, Pingyu Zhang, Ping Qiu, Weixin Xie, Dong Wang, Ben Zhong Tang, Ke Wang

Advanced Functional Materials· Volume 33· Issue 46
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Source abstract

Abstract

Abstract The limited tissue penetration depth and spatial resolution are the major bottlenecks for deep‐brain imaging. In this study, molecular engineering by tailoring electron donors is conducted to develop for the first time an NIR‐II (second near‐infrared) emissive fluorescence probe, namely DCTBT, for effective deep‐brain two‐photon fluorescence imaging. Benefiting from its good biocompatibility, high photostability, bright NIR‐II emission as aggregates and large two‐photon fluorescence action cross section at the 1700 nm excitation window, DCTBT offers the imaging depths of 2180 and 1135 µm in mouse brain with removed and intact skull, respectively. These results are the record depths for brain imaging, compared to all kinds of fluorescent probes and all modalities of multiphoton microscopy at all demonstrated excitation wavelengths. Moreover, with DCTBT labeling, hemodynamic imaging of blood flow in mouse brain vessels down to a depth of 714 µm with the intact skull is achieved. Multiphoton fluorescence imaging with the NIR‐II probe DCTBT excited at the 1700 nm window may readily provide methodology for deep‐brain structural and hemodynamic research.
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Research context

Biomaterials

OpenAlex topics: Nanoplatforms for cancer theranostics · Luminescence and Fluorescent Materials · Advanced Fluorescence Microscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center for AIE Research Shenzhen Key Laboratory of Polymer Science and Technology Guangdong Research Center for Interfacial Engineering of Functional Materials College of Material Science and Engineering Shenzhen University Shenzhen 518060 China

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060 China

College of Chemistry and Environmental Engineering Shenzhen University Shenzhen 518060 China

Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging School of Biomedical Engineering Health Science Center Shenzhen University Shenzhen 518060 China

College of Electronics and Information Engineering Shenzhen University Shenzhen 518060 China

Shenzhen Institute of Molecular Aggregate Science and Engineering School of Science and Engineering The Chinese University of Hong Kong Shenzhen Shenzhen 518172 China

Funding

National Natural Science Foundation of China · China Postdoctoral Science Foundation

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