Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
Source abstract
Abstract
Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper.
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Research context
OpenAlex topics: RNA Research and Splicing · RNA and protein synthesis mechanisms · RNA modifications and cancer
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA
Department of Neurology and Rehabilitation Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
Structural Studies Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
Research Department of Cell and Developmental Biology, UCL Division of Biosciences, London WC1E 6DE, UK
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