Biomimetic GelMPC Micropatterns on Titanium and Their Effects on Platelets and Endothelialization

Currently, poly(2‐Methacryloyloxyethyl phosphorylcholine) (PMPC) is widely used for surface modification of biomaterials, but micropatterned immobilization of PMPC on Ti is not so common. To establish micropattern for improving hemocompatibility and orderly endothelialization, gelatin, which has good endothelial cell compatibility, is chosen to crosslink with PMPC. GelMPC is synthesized with gelatin and poly(MPC‐co‐methacrylic acid) (PMMPC) via condensation reaction. GelMPC is immobilized on Ti with micropatterned PDMS stamp. FTIR, SEM, and water contact angle demonstrate that the GelMPC micropattern is fabricated successfully. Platelets on GelMPC micropattern are round and not activated. Endothelial cells and smooth muscle cells cultured on GelMPC micropattern show the cells are aligned in the direction of the pattern, simultaneously the NO release of ECs is upregulated and the number of ECs on GelMPC micropattern increase after 3 days’ culture. However, the number of SMCs on GelMPC micropattern is smaller than that on unmodified (Ti and Ti‐PDA) after 1 day and 3 days’ culture. The authors believe that the method will provide a potential solution for regulation of ECs and SMCs and endothelialization of cardiovascular materials.